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1 ml of microbial culture was diluted to 10ml and 1ml of diluted culture was further diluted by adding 99ml of buffer. 0.5 ml of final culture was plated on to an agar plate. Then, 200 colonies were observed. What was the number of bacteria per ml in the original culture?
- a)1 × 105
- b)2 × 104
- c)2 × 105
- d)4 × 105
Correct answer is option 'D'. Can you explain this answer?
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Verified Answer
1 ml of microbial culture was diluted to 10ml and 1ml of diluted cultu...
Dilution factor
- 1 ml original diluted to 10ml means 10 times dilution
- 1 ml of diluted culture further diluted to 100 ml means 100 times dilution
Total dilution factor = 1000 times = 10-3
Here. 0.5 ml of final culture contains 200 colonies
Most Upvoted Answer
1 ml of microbial culture was diluted to 10ml and 1ml of diluted cultu...
To find the number of bacteria per ml in the original culture, we can use the dilution formula:
C1V1 = C2V2
Where:
C1 = concentration of original culture
V1 = volume of original culture
C2 = concentration of final culture
V2 = volume of final culture
In the first dilution step, 1 ml of microbial culture was diluted to 10 ml. Therefore, the concentration of the final culture (C2) is 1/10 = 0.1 times the concentration of the original culture (C1).
In the second dilution step, 1 ml of the diluted culture was further diluted by adding 99 ml of buffer. Therefore, the concentration of the final culture (C2) is 1/100 = 0.01 times the concentration of the diluted culture (C1).
Now we have all the information we need to calculate the concentration of the original culture:
C1V1 = C2V2
C1 * 1 ml = 0.01 * 100 ml
C1 = 1 bacteria/ml
Therefore, the number of bacteria per ml in the original culture is 1.
C1V1 = C2V2
Where:
C1 = concentration of original culture
V1 = volume of original culture
C2 = concentration of final culture
V2 = volume of final culture
In the first dilution step, 1 ml of microbial culture was diluted to 10 ml. Therefore, the concentration of the final culture (C2) is 1/10 = 0.1 times the concentration of the original culture (C1).
In the second dilution step, 1 ml of the diluted culture was further diluted by adding 99 ml of buffer. Therefore, the concentration of the final culture (C2) is 1/100 = 0.01 times the concentration of the diluted culture (C1).
Now we have all the information we need to calculate the concentration of the original culture:
C1V1 = C2V2
C1 * 1 ml = 0.01 * 100 ml
C1 = 1 bacteria/ml
Therefore, the number of bacteria per ml in the original culture is 1.
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1 ml of microbial culture was diluted to 10ml and 1ml of diluted culture was further diluted by adding 99ml of buffer. 0.5 ml of final culture was plated on to an agar plate. Then, 200 colonies were observed. What was the number of bacteria per ml in the original culture?a)1 × 105b)2 × 104c)2 × 105d)4 × 105Correct answer is option 'D'. Can you explain this answer?
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1 ml of microbial culture was diluted to 10ml and 1ml of diluted culture was further diluted by adding 99ml of buffer. 0.5 ml of final culture was plated on to an agar plate. Then, 200 colonies were observed. What was the number of bacteria per ml in the original culture?a)1 × 105b)2 × 104c)2 × 105d)4 × 105Correct answer is option 'D'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about 1 ml of microbial culture was diluted to 10ml and 1ml of diluted culture was further diluted by adding 99ml of buffer. 0.5 ml of final culture was plated on to an agar plate. Then, 200 colonies were observed. What was the number of bacteria per ml in the original culture?a)1 × 105b)2 × 104c)2 × 105d)4 × 105Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for 1 ml of microbial culture was diluted to 10ml and 1ml of diluted culture was further diluted by adding 99ml of buffer. 0.5 ml of final culture was plated on to an agar plate. Then, 200 colonies were observed. What was the number of bacteria per ml in the original culture?a)1 × 105b)2 × 104c)2 × 105d)4 × 105Correct answer is option 'D'. Can you explain this answer?.
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