There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72andordm;C, Anealing 45andordm;C, Extension 92andordm;Cb)Denaturation 94anddeg;C,Anealing = Tmandplusmn;4anddeg;. Extension 72anddeg;Cc)Denaturation and Extension 94anddeg;C and Anealing = Tmandplusmn;4anddeg;d)Denaturation 94anddeg;C,Anealing = Tmandplusmn;4anddeg;. Extension 4anddeg;CCorrect answer is option 'B'. Can you explain this answer?

EduRev

All Courses

Explore Courses UPSC Exam UPSC Test Series Free Notes EduRev Infinity

Open App

IIT JAM Exam > IIT JAM Questions > There is mention the temperature of different...

Join the Discussion on the App

There is mention the temperature of different steps of PCR, find out the correct match

a)
Denaturation 72ºC, Anealing 45ºC, Extension 92ºC
b)
Denaturation 94°C, Anealing = T_m±4°. Extension 72°C
c)
Denaturation and Extension 94°C' and Anealing = T_m±4°
d)
Denaturation 94°C, Anealing = T_m±4°. Extension 4°C

Correct answer is option 'B'. Can you explain this answer?

FREE This question is part of

Techniques MCQ

Attempt Test

Attempt this Test

Verified Answer

There is mention the temperature of different steps of PCR, find out t...

PCR is a technique which is use to amplify DNA invitro. It involves following steps.
(i) Denaturation at 94/95°C
(ii) Annealing at T_m± 4°
(iii) Extension at 72°C

View all questions of this test

Most Upvoted Answer

There is mention the temperature of different steps of PCR, find out t...

B)Annealing 95
c)Extension 55

View all answers Start learning for free

< Previous Question Next Question >

Explore Courses for IIT JAM exam

Similar IIT JAM Doubts

Find the correct option from the following options

View answer

Amplification of a DNA fragment by PCR yields only one faint band of the expected size on an agarose gel. For such a sample, the best way to increase the yield of the PCR product is to

View answer

A protein P can exist in either the native form N or the denatured form D. A ligand L binds to N but not to D. Thermal denaturation of P was monitored both in presence and in absence of L.Which one of the following statements holds true for these denaturation experiments?

View answer

Under identical conditions of assay, an enzyme from E. coli converts 45% of a substrate to product whereas the same enzyme from yeast converts 66% of the substrate.The following explanations were put forward as possible reasons to explain this difference:P. The equilibrium constant are different.Q. The rates of the reactions are different.S. The temperature for optimal activity are different.Which of the above three are likely to be true?

View answer

Which of the following statement is true

View answer

There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer?

Share with a Friend

Answer this doubt

Question Description
There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer?.

Solutions for There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer? in English & in Hindi are available as part of our courses for IIT JAM. Download more important topics, notes, lectures and mock test series for IIT JAM Exam by signing up for free.

Here you can find the meaning of There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer?, a detailed solution for There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer? has been provided alongside types of There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer? theory, EduRev gives you an ample number of questions to practice There is mention the temperature of different steps of PCR, find out the correct matcha)Denaturation 72ºC, Anealing 45ºC, Extension 92ºCb)Denaturation 94°C,Anealing = Tm±4°. Extension 72°Cc)Denaturation and Extension 94°C and Anealing = Tm±4°d)Denaturation 94°C,Anealing = Tm±4°. Extension 4°CCorrect answer is option 'B'. Can you explain this answer? tests, examples and also practice IIT JAM tests.

Explore Courses for IIT JAM exam