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A research scholar has a mixture of proteins with the following properties.
Protein P → M.wt = 11 Kd; PI = 10
Protein Q →M.wt = 60 Kd: PI = 4
Protein R →M.wt = 26 Kd: PI = 8
Protein S→M.wt = 9 Kd; PI = 5
Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.
- a)P, R, S, Q
- b)Q, P, R, S
- c)Can not be predicted
- d)S, P, R, Q
Correct answer is option 'A'. Can you explain this answer?
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Verified Answer
A research scholar has a mixture of proteins with the following proper...
As DEAE - cellulose is used that means fixed ion is positive charge in ion exchange chromatogram. So negative charge ions will be attracted and elute later.
So the P will elute first and then R and then S and finally Q.
Most Upvoted Answer
A research scholar has a mixture of proteins with the following proper...
- Soluble in water
- Basic in nature
- Contains a large number of positively charged amino acids
Protein Q
- Insoluble in water
- Acidic in nature
- Contains a large number of negatively charged amino acids
Protein R
- Soluble in water
- Neutral in nature
- Contains an equal number of positively and negatively charged amino acids
Protein S
- Insoluble in water
- Neutral in nature
- Contains an equal number of positively and negatively charged amino acids
Based on these properties, the research scholar can use various techniques to separate and purify these proteins. Here are some possible methods for each protein:
Protein P:
- Since it is soluble in water, it can be purified using techniques like dialysis or gel filtration chromatography. These methods exploit the solubility of the protein to separate it from other components in the mixture.
- The basic nature of Protein P suggests that it can also be purified using ion exchange chromatography, where the positively charged amino acids in the protein can be selectively bound to a negatively charged column. Elution with a salt gradient can then release the protein from the column.
Protein Q:
- As Protein Q is insoluble in water, methods like salting out or precipitation can be used to separate it from the mixture. These techniques exploit the differences in solubility between the protein and other components.
- The acidic nature of Protein Q suggests that it can be purified using ion exchange chromatography, similar to Protein P. In this case, the negatively charged amino acids in the protein can be selectively bound to a positively charged column.
Protein R and Protein S:
- Both Protein R and Protein S have similar properties and can be purified using similar techniques.
- Since they are soluble in water, dialysis or gel filtration chromatography can be used to separate them from other components.
- The neutral nature of these proteins suggests that techniques like size exclusion chromatography or hydrophobic interaction chromatography can be used for further purification. These methods exploit differences in size or hydrophobicity between proteins to separate them.
Overall, the research scholar can utilize a combination of these techniques to separate and purify each protein based on their unique properties.
- Basic in nature
- Contains a large number of positively charged amino acids
Protein Q
- Insoluble in water
- Acidic in nature
- Contains a large number of negatively charged amino acids
Protein R
- Soluble in water
- Neutral in nature
- Contains an equal number of positively and negatively charged amino acids
Protein S
- Insoluble in water
- Neutral in nature
- Contains an equal number of positively and negatively charged amino acids
Based on these properties, the research scholar can use various techniques to separate and purify these proteins. Here are some possible methods for each protein:
Protein P:
- Since it is soluble in water, it can be purified using techniques like dialysis or gel filtration chromatography. These methods exploit the solubility of the protein to separate it from other components in the mixture.
- The basic nature of Protein P suggests that it can also be purified using ion exchange chromatography, where the positively charged amino acids in the protein can be selectively bound to a negatively charged column. Elution with a salt gradient can then release the protein from the column.
Protein Q:
- As Protein Q is insoluble in water, methods like salting out or precipitation can be used to separate it from the mixture. These techniques exploit the differences in solubility between the protein and other components.
- The acidic nature of Protein Q suggests that it can be purified using ion exchange chromatography, similar to Protein P. In this case, the negatively charged amino acids in the protein can be selectively bound to a positively charged column.
Protein R and Protein S:
- Both Protein R and Protein S have similar properties and can be purified using similar techniques.
- Since they are soluble in water, dialysis or gel filtration chromatography can be used to separate them from other components.
- The neutral nature of these proteins suggests that techniques like size exclusion chromatography or hydrophobic interaction chromatography can be used for further purification. These methods exploit differences in size or hydrophobicity between proteins to separate them.
Overall, the research scholar can utilize a combination of these techniques to separate and purify each protein based on their unique properties.
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A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer?
Question Description
A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer?.
A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer?.
Solutions for A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? in English & in Hindi are available as part of our courses for IIT JAM.
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Here you can find the meaning of A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of
A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer?, a detailed solution for A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? has been provided alongside types of A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? tests, examples and also practice IIT JAM tests.
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