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A research scholar has a mixture of proteins with the following properties.
Protein P → M.wt = 11 Kd; PI = 10
Protein Q →M.wt = 60 Kd: PI = 4
Protein R →M.wt = 26 Kd: PI = 8
Protein S→M.wt = 9 Kd; PI = 5
Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.
  • a)
    P, R, S, Q
  • b)
    Q, P, R, S
  • c)
    Can not be predicted
  • d)
    S, P, R, Q
Correct answer is option 'A'. Can you explain this answer?
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A research scholar has a mixture of proteins with the following proper...

As DEAE - cellulose is used that means fixed ion is positive charge in ion exchange chromatogram. So negative charge ions will be attracted and elute later.
So the P will elute first and then R and then S and finally Q.
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Most Upvoted Answer
A research scholar has a mixture of proteins with the following proper...
- Soluble in water
- Basic in nature
- Contains a large number of positively charged amino acids

Protein Q
- Insoluble in water
- Acidic in nature
- Contains a large number of negatively charged amino acids

Protein R
- Soluble in water
- Neutral in nature
- Contains an equal number of positively and negatively charged amino acids

Protein S
- Insoluble in water
- Neutral in nature
- Contains an equal number of positively and negatively charged amino acids

Based on these properties, the research scholar can use various techniques to separate and purify these proteins. Here are some possible methods for each protein:

Protein P:
- Since it is soluble in water, it can be purified using techniques like dialysis or gel filtration chromatography. These methods exploit the solubility of the protein to separate it from other components in the mixture.
- The basic nature of Protein P suggests that it can also be purified using ion exchange chromatography, where the positively charged amino acids in the protein can be selectively bound to a negatively charged column. Elution with a salt gradient can then release the protein from the column.

Protein Q:
- As Protein Q is insoluble in water, methods like salting out or precipitation can be used to separate it from the mixture. These techniques exploit the differences in solubility between the protein and other components.
- The acidic nature of Protein Q suggests that it can be purified using ion exchange chromatography, similar to Protein P. In this case, the negatively charged amino acids in the protein can be selectively bound to a positively charged column.

Protein R and Protein S:
- Both Protein R and Protein S have similar properties and can be purified using similar techniques.
- Since they are soluble in water, dialysis or gel filtration chromatography can be used to separate them from other components.
- The neutral nature of these proteins suggests that techniques like size exclusion chromatography or hydrophobic interaction chromatography can be used for further purification. These methods exploit differences in size or hydrophobicity between proteins to separate them.

Overall, the research scholar can utilize a combination of these techniques to separate and purify each protein based on their unique properties.
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Explanation:The separation techniques of proteins are used to isolate and purify proteins from complex mixtures. These techniques are based on various properties of proteins such as solubility, charge, size, and others. However, the technique of protein separation based on viscosity is not commonly used. Protein solubility:Protein solubility refers to the ability of a protein to dissolve in a given solvent. Different proteins have different solubilities in different solvents. This property is often used in techniques like precipitation, where proteins are selectively precipitated out of solution based on their solubility.Protein charge:Protein charge refers to the net charge of a protein molecule, which is determined by the presence of charged amino acid residues such as lysine, arginine, aspartic acid, and glutamic acid. Techniques like electrophoresis and ion exchange chromatography utilize the charge differences between proteins to separate them.Protein size:Protein size refers to the molecular weight or size of a protein molecule. Techniques such as gel filtration chromatography and SDS-PAGE separate proteins based on their size. In gel filtration chromatography, proteins are separated based on their ability to enter and pass through a porous gel matrix, while in SDS-PAGE, proteins are separated based on their migration through a gel under the influence of an electric field.Protein viscosity:Protein viscosity refers to the resistance of a protein solution to flow. While protein viscosity can vary depending on factors such as protein concentration, temperature, and pH, it is not typically used as a property for protein separation. In conclusion, the separation techniques of proteins are based on properties such as solubility, charge, and size. Protein viscosity is not commonly used as a property for protein separation.

A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer?
Question Description
A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A research scholar has a mixture of proteins with the following properties.Protein P →M.wt = 11 Kd; PI = 10Protein Q →M.wt = 60 Kd: PI = 4Protein R →M.wt = 26 Kd: PI = 8Protein S→M.wt = 9 Kd; PI = 5Predict the order of elution of these proteins when a mixture of the four proteins is separated by DEAE-cellulose chromatography at pH 7.a)P, R, S, Qb)Q, P, R, Sc)Can not be predictedd)S, P, R, QCorrect answer is option 'A'. Can you explain this answer?.
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