Solution Concentration Calculation:

To calculate the solution concentration, we need to use the Beer-Lambert Law, which relates the absorbance of a sample to its concentration and the molar absorptivity.

The Beer-Lambert Law is given by the equation:

A = εlc

Where:
A is the absorbance
ε is the molar absorptivity (ε2801mg/m γG = 1.4)
l is the path length (in cm)
c is the concentration (in mol/L)

We are given the A280nm reading for a 1/200 dilution, which is 0.83.

The dilution factor is 1/200, which means the concentration of the original sample can be calculated by multiplying the dilution factor by the absorbance:

c = (A × dilution factor) / ε

Substituting the given values, we get:

c = (0.83 × 1/200) / 1.4 = 0.000297 mol/L

Therefore, the solution concentration is 0.000297 mol/L.

Total Protein Calculation:

To calculate the total protein, we need to multiply the solution concentration by the volume of the sample.

Given that the volume of the sample is 175 μl (or 0.175 ml), we can calculate the total protein as follows:

Total protein = solution concentration × volume of the sample

Total protein = 0.000297 mol/L × 0.175 ml

Total protein = 0.0000519 mol

Since we know the molecular weight of γG, we can convert the total protein from moles to grams:

Total protein (in grams) = Total protein (in moles) × molecular weight

Assuming the molecular weight of γG is 150 g/mol (just for example), we can calculate the total protein as follows:

Total protein (in grams) = 0.0000519 mol × 150 g/mol

Total protein (in grams) = 0.007785 g

Therefore, the calculated solution concentration is 0.000297 mol/L and the total protein is 0.007785 g.