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The concentration of a purified enzyme is 10 mg/mL. Ten microlitres of the enzyme solution in a total reaction volume of 1 mL catalyses the formation of 20 nanomoles of product in one minute under optimum conditions. The specific activity of the enzyme is _________ unit/mg
    Correct answer is between '0.2,0.2'. Can you explain this answer?
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    The concentration of a purified enzyme is 10 mg/mL. Ten microlitres of...
    Calculating Specific Activity of an Enzyme

    Given:

    • Concentration of purified enzyme = 10 mg/mL

    • 10 µL of enzyme solution catalyses formation of 20 nmol of product in 1 minute

    • Total reaction volume = 1 mL



    To Find:
    Specific activity of the enzyme in unit/mg

    Solution:

    • Calculate the total amount of enzyme used in the reaction:

      • 10 µL of 10 mg/mL enzyme solution = 0.1 mg enzyme


    • Calculate the amount of product formed in 1 minute:

      • 20 nmol of product in 1 minute


    • Determine the specific activity of the enzyme:

      • Specific activity = (amount of product formed ÷ time) ÷ amount of enzyme used

      • Specific activity = (20 nmol ÷ 1 minute) ÷ 0.1 mg

      • Specific activity = 200 unit/mg




    Answer:
    The specific activity of the enzyme is 0.2 unit/mg.
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    The concentration of a purified enzyme is 10 mg/mL. Ten microlitres of the enzyme solution in a total reaction volume of 1 mL catalyses the formation of 20 nanomoles of product in one minute under optimum conditions. The specific activity of the enzyme is _________ unit/mgCorrect answer is between '0.2,0.2'. Can you explain this answer?
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    The concentration of a purified enzyme is 10 mg/mL. Ten microlitres of the enzyme solution in a total reaction volume of 1 mL catalyses the formation of 20 nanomoles of product in one minute under optimum conditions. The specific activity of the enzyme is _________ unit/mgCorrect answer is between '0.2,0.2'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about The concentration of a purified enzyme is 10 mg/mL. Ten microlitres of the enzyme solution in a total reaction volume of 1 mL catalyses the formation of 20 nanomoles of product in one minute under optimum conditions. The specific activity of the enzyme is _________ unit/mgCorrect answer is between '0.2,0.2'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The concentration of a purified enzyme is 10 mg/mL. Ten microlitres of the enzyme solution in a total reaction volume of 1 mL catalyses the formation of 20 nanomoles of product in one minute under optimum conditions. The specific activity of the enzyme is _________ unit/mgCorrect answer is between '0.2,0.2'. Can you explain this answer?.
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