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A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes:
| | Father | Mother | Offspring I | Offspring II |
| First probe | + | + | - | + |
| Second probe | + | + | + | - |
On the basis of the result, which of the following statements is correct?
- a)Parents are affected
- b)Offspring I is affected
- c)Offspring II is carrier
- d)Offspring II is affected
Correct answer is option 'B'. Can you explain this answer?
Verified Answer
A technique called allele-specific hybridization is used to find the m...
Concept:
- Allele-specific hybridization is a technique based on discriminating between alleles at SNP using allele-specific oligonucleotide (ASO) probes and exploits the 5′ exonuclease activity of DNA polymerases.
- Allele-specific PCR (AS-PCR) is achieved by PCR by an allele-specific primer rather than direct detection by a probe. Three primers are required, two to match the two alleles at the SNP site and the third to anneal to the opposite strand.
Explanation:
Fig 2: Principles of allele-specific polymerase chain reaction (PCR) as an allele discrimination method
- Allele-specific polymerase chain reaction (PCR) is an extension of the allele-specific hybridization principle, in which discrimination is achieved by allele-specific primer annealing followed by PCR amplification rather than the direct detection of a hybridized probe.
- Three primers are required, two to match alternative alleles at the SNP site and one to anneal to the opposite strand.
- Two reactions are set up, each containing one of the SNP primers together with the reverse primer.
- Although the variable base position can be sited somewhere in the middle of the SNP primers, in a manner directly analogous to the ASO strategy
- This method tends to allow the extension of a mismatched primer–template because the stringency of annealing cannot be optimized (annealing has to favor both primers in the reaction and is therefore difficult to optimize for the allele-specific primer alone).
- The preferred approach is to place the 3′ end of one of the primers at the variable base position since extension is dependent on perfect complementarity at the 3′ end of the primer.
- Now the probe used here is TGACTCCTGAGGAGAAGTC and TGACTCCTGTGGAGAAGTC so from the sequence of probe one thing is clear that probe 2 binds to the affected individual.
- from the figure it is clear that since probe 2 is detected in offspring I its is the affected one.
hence the correct answer is option 2
Most Upvoted Answer
A technique called allele-specific hybridization is used to find the m...
Explanation:
Allele-Specific Hybridization Technique:
- This technique is used to detect specific mutations in DNA samples by probing with specific oligonucleotides.
Analysis of Results:
- When the first probe was used, both parents and Offspring II showed a signal, but Offspring I did not.
- When the second probe was used, both parents and Offspring I showed a signal, but Offspring II did not.
Interpretation:
- Since Offspring I showed a signal with the second probe but not with the first probe, it indicates that Offspring I carries the mutation (GAG - GTG).
- Offspring II did not show a signal with the second probe, suggesting that Offspring II does not carry the mutation.
Therefore, based on the results obtained from the allele-specific hybridization technique, it can be concluded that Offspring I is affected by the mutation (GAG - GTG).
Allele-Specific Hybridization Technique:
- This technique is used to detect specific mutations in DNA samples by probing with specific oligonucleotides.
Analysis of Results:
- When the first probe was used, both parents and Offspring II showed a signal, but Offspring I did not.
- When the second probe was used, both parents and Offspring I showed a signal, but Offspring II did not.
Interpretation:
- Since Offspring I showed a signal with the second probe but not with the first probe, it indicates that Offspring I carries the mutation (GAG - GTG).
- Offspring II did not show a signal with the second probe, suggesting that Offspring II does not carry the mutation.
Therefore, based on the results obtained from the allele-specific hybridization technique, it can be concluded that Offspring I is affected by the mutation (GAG - GTG).
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A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer?
Question Description
A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer?.
A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer?.
Solutions for A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? in English & in Hindi are available as part of our courses for Software Development.
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Here you can find the meaning of A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of
A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer?, a detailed solution for A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? has been provided alongside types of A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice A technique called allele-specific hybridization is used to find the mutation (GAG - GTG). A family with four members is the subject of an investigation. After the proper processing, DNA from the parents' and their two children's blood samples is spotted on a membrane and, after labelling, is probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe). Signals are collected for the locations where DNA from the parents and offspring II are spotted while the first oligonucleotide is being probed. Signals are obtained at the positions where DNA from the parents and offspring I when the second oligonucleotide was probed. Below are the outcomes: Father Mother Offspring I Offspring II First probe + + - + Second probe + + + - On the basis of the result, which of the following statements is correct?a)Parents are affectedb)Offspring I is affectedc)Offspring II is carrierd)Offspring II is affectedCorrect answer is option 'B'. Can you explain this answer? tests, examples and also practice Software Development tests.
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