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Lac Operon Video Lecture | Biology Class 12 - NEET

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FAQs on Lac Operon Video Lecture - Biology Class 12 - NEET

1. What is the lac operon?
The lac operon is a group of genes in bacteria that are involved in the metabolism of lactose. It consists of three main components: the lacZ gene, which encodes the enzyme β-galactosidase; the lacY gene, which encodes the lactose permease; and the lacA gene, which encodes the transacetylase enzyme. These genes are controlled by a promoter and an operator region, which determine their expression.
2. How does the lac operon work?
The lac operon is regulated by a system of inducible control. In the absence of lactose, a repressor protein binds to the operator region and prevents the expression of the lac genes. When lactose is present, it binds to the repressor protein, causing a conformational change that releases the repressor from the operator. This allows RNA polymerase to bind to the promoter region and transcribe the lac genes.
3. What is the role of the lacZ gene in the lac operon?
The lacZ gene encodes the enzyme β-galactosidase, which is responsible for the hydrolysis of lactose into glucose and galactose. This enzyme is crucial for the metabolism of lactose by the bacteria. Without the lacZ gene, the bacteria would be unable to break down lactose and utilize it as a source of energy.
4. How does the lac operon respond to glucose levels?
The lac operon exhibits a phenomenon known as catabolite repression, which means that its expression is suppressed in the presence of high levels of glucose. When glucose is available, the concentration of cyclic AMP (cAMP), which is required for the activation of the lac operon, decreases. As a result, the lac operon is less likely to be transcribed even in the presence of lactose.
5. What are the practical applications of studying the lac operon?
Studying the lac operon has several practical applications in the field of molecular biology. It has provided valuable insights into gene regulation and the mechanisms of inducible control. The lac operon is often used as a model system for studying gene expression and regulation. Additionally, the lacZ gene, which encodes β-galactosidase, is widely used as a reporter gene in molecular biology experiments to assess gene expression.
78 videos|276 docs|174 tests
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