Q1: Answer the following questions in about 150 words each : (10x5=50)
(a) Enumerate the functions of Endoplasmic reticulum and Golgi apparatus. (10 Marks)
Ans:
Introduction:
The endoplasmic reticulum (ER) and Golgi apparatus are essential cellular organelles involved in the synthesis, modification, and transport of proteins and lipids.
Endoplasmic Reticulum (ER):
Protein Synthesis: The rough ER, studded with ribosomes, is responsible for synthesizing proteins that are destined for secretion or insertion into membranes.
Lipid Synthesis: The smooth ER is involved in the synthesis of lipids, including phospholipids and cholesterol.
Protein Folding and Modification: The ER provides a controlled environment for proper folding and post-translational modifications of proteins.
Detoxification: The smooth ER in liver cells detoxifies drugs and harmful substances by metabolizing them into water-soluble forms.
Calcium Storage: The ER stores and regulates calcium ion levels, which are crucial for various cellular processes.
Golgi Apparatus:
Protein Modification and Sorting: The Golgi apparatus receives proteins from the ER and modifies them by adding sugars or other groups. It also sorts and packages proteins into vesicles for transport.
Lipid Metabolism: It plays a role in lipid metabolism, modifying and sorting lipids for cellular use or export.
Formation of Lysosomes: The Golgi apparatus synthesizes lysosomal enzymes and packages them into vesicles called lysosomes.
Cell Wall Formation (in plant cells): In plant cells, the Golgi apparatus is involved in synthesizing components of the cell wall, such as cellulose.
Secretion of Cellulose in Some Protists: In certain protists, the Golgi apparatus secretes cellulose, which forms protective coverings.
Conclusion:
The endoplasmic reticulum and Golgi apparatus work in tandem to ensure proper synthesis, modification, and transport of proteins and lipids within the cell, playing critical roles in cellular function.
(b) Explain inbreeding depression and its effect in crops. Also explain the degrees of inbreeding depression. (10 Marks)
Ans:
Introduction:
Inbreeding depression occurs when closely related individuals are bred together over several generations, leading to a decline in the fitness and productivity of the offspring.
Effects of Inbreeding Depression:
Reduced Vigor and Growth: Offspring of closely related parents may exhibit slower growth rates and reduced overall vigor.
Increased Susceptibility to Diseases: Weakened immune systems and genetic vulnerabilities make inbred plants more susceptible to diseases and pests.
Decreased Reproductive Success: Inbred plants may produce fewer viable seeds or have lower pollen viability, reducing reproductive success.
Loss of Heterozygosity: Inbreeding leads to the loss of genetic diversity, as offspring become increasingly homozygous for deleterious alleles.
Degrees of Inbreeding Depression:
First-Degree Inbreeding: Offspring result from the mating of siblings or parent-offspring pairs. This leads to the highest risk of inbreeding depression.
Second-Degree Inbreeding: Offspring result from the mating of first cousins. The risk of inbreeding depression is lower compared to first-degree inbreeding.
Third-Degree Inbreeding and Beyond: As the degree of relatedness decreases, so does the risk of inbreeding depression. However, some level of risk remains.
Example: Inbreeding depression can be observed in self-pollinating crops like wheat. Continuous self-pollination over generations can lead to reduced yields and poor plant health due to accumulated genetic defects.
Conclusion:
Managing inbreeding is crucial in crop breeding programs to maintain genetic diversity and prevent the negative effects of inbreeding depression.
(c) Briefly discuss the requirements for production of certified seeds. (10 Marks)
Ans:
Introduction:
Certified seeds are high-quality seeds that meet specific standards of purity, germination, and genetic identity. Their production involves several key requirements.
Requirements:
Genetic Purity: Certified seeds must possess genetic purity, meaning they should be true-to-type and free from genetic contamination.
Physical Purity: Seeds should be free from impurities like other crop seeds, weed seeds, and inert matter.
Germination Standards: Certified seeds must meet or exceed specified germination standards to ensure high and uniform germination rates.
Field Inspection: Fields where certified seeds are produced must undergo regular inspections to ensure compliance with quality standards.
Isolation Distance: Adequate isolation distances must be maintained to prevent cross-pollination with non-certified crops of the same species.
Rogueing and Rouging: Removal of off-types and impurities from seed crops during the growing season.
Example: In the production of certified wheat seeds, farmers must maintain sufficient isolation distances from other wheat varieties to prevent cross-pollination. Field inspections are conducted to verify compliance with quality standards.
Conclusion:
Production of certified seeds requires strict adherence to quality standards to ensure that the seeds are of high genetic and physical purity, promoting better crop performance and yield potential.
(d) Give the classification of soil water. Briefly discuss soil moisture availability with respect to soil type. (10 Marks)
Ans:
Introduction:
Soil water is classified based on its availability to plants, which is crucial for determining irrigation and water management strategies.
Classification of Soil Water:
Hygroscopic Water: Held tightly by soil particles and is not available for plant use. Plants cannot extract this water.
Capillary Water: Held in soil capillaries and is available for plant uptake. This is the water that plants can access between field capacity and wilting point.
Gravitational Water: Excess water that drains below the root zone due to gravity. It is not available for plant use.
Soil Moisture Availability with Respect to Soil Type:
Sandy Soils: Have larger particles, allowing water to drain quickly. They have lower water-holding capacity and are prone to drought stress.
Loam Soils: Have a balanced mixture of sand, silt, and clay particles. They retain moisture well and are considered ideal for most crops.
Clay Soils: Have fine particles that hold water tightly. They have high water-holding capacity but can be prone to waterlogging.
Example: In a sandy soil, water drains rapidly, making it necessary to irrigate more frequently. In contrast, a loam soil retains moisture effectively, requiring less frequent irrigation.
Conclusion:
Understanding soil water classification and moisture availability based on soil type is crucial for making informed decisions regarding irrigation, crop selection, and water management practices.
(e) Write down about the origin and domestication of rice. (10 Marks)
Ans:
Introduction:
Rice (Oryza sativa) is one of the world's most important staple crops, providing a significant portion of global food consumption. Its origin and domestication have a rich history.
Origin:
Domestication:
Example of Domestication:
Spread:
Cultural Significance:
Conclusion:
The origin and domestication of rice represent a critical milestone in the history of agriculture. Its cultivation has had profound impacts on societies, economies, and cultures across Asia and beyond.
Q2:
(a) Enlist the molecular models of cell membrane and explain the models given by S.J. Singer and G. Nicholson (1972), Green and Capaldi (1974) and Racker (1976). (20 Marks)
Ans:
Introduction:
Cell membranes are dynamic structures composed of lipids and proteins. Several molecular models have been proposed to describe the organization of cell membranes.
S.J. Singer and G. Nicholson Model (1972):
Green and Capaldi Model (1974):
Racker Model (1976):
Conclusion:
These models have contributed to our understanding of cell membrane structure and function. While the Fluid Mosaic Model remains widely accepted, the other models have provided valuable insights into specific aspects of membrane organization.
(b) Give an account of seven different pairs of contrasting characters whose inheritance was studied by Mendel in Garden Pea (Pisum sativum). Give the reasons for Mendel’s success in the study. (20 Marks)
Ans:
Introduction:
Gregor Mendel's experiments with garden peas laid the foundation for modern genetics. He studied seven pairs of contrasting characters in pea plants.
Contrasting Characters Studied by Mendel:
Seed Color (Yellow vs. Green): Yellow (dominant) vs. green (recessive).
Seed Texture (Smooth vs. Wrinkled): Smooth (dominant) vs. wrinkled (recessive).
Flower Color (Purple vs. White): Purple (dominant) vs. white (recessive).
Flower Position (Axial vs. Terminal): Axial (dominant) vs. terminal (recessive).
Stem Length (Long vs. Short): Long (dominant) vs. short (recessive).
Pod Color (Yellow vs. Green): Yellow (dominant) vs. green (recessive).
Pod Shape (Inflated vs. Constricted): Inflated (dominant) vs. constricted (recessive).
Reasons for Mendel's Success:
Selection of Suitable Organism: Pea plants (Pisum sativum) were ideal for Mendel's experiments due to their distinct traits, easy cultivation, and controlled pollination.
Purity of Parental Lines: Mendel ensured that the plants used were true-breeding for specific traits, reducing variability in offspring.
Large Sample Size: Mendel performed extensive experiments with thousands of plants, providing robust statistical data.
Isolation of Crosses: Mendel performed controlled crosses, preventing unintended pollination from other plants.
Mathematical Analysis: Mendel applied quantitative analysis to his data, introducing principles of probability and statistics.
Conclusion:
Mendel's careful experimental design, selection of traits, and rigorous data analysis were instrumental in his groundbreaking discoveries in genetics.
(c) Explain various theories of inheritance. Write down the evidences suggesting the presence of cytoplasmic inheritance in the crosses’ results. (10 Marks)
Ans:
Introduction:
Various theories have been proposed to explain the transmission of genetic traits. Cytoplasmic inheritance involves the transmission of genetic information through cytoplasmic organelles.
Theories of Inheritance:
1. Blending Inheritance:
2. Particulate Inheritance (Mendelian Inheritance):
3. Chromosomal Theory of Inheritance:
Cytoplasmic Inheritance:
Conclusion:
While Mendelian inheritance primarily involves nuclear DNA, cytoplasmic inheritance plays a role in transmitting genetic information through organelles in the cytoplasm. This is particularly evident in traits linked to mitochondria and chloroplasts.
Q3:
(a) Describe the history of plant breeding in India. Write the objectives of plant breeding and methods for creation of variability for crop improvement. (20 Marks)
Ans:
Introduction:
Plant breeding in India has a rich history dating back thousands of years. It has evolved from traditional practices to modern scientific methods.
Ancient and Traditional Practices (Pre-Independence):
Selection and Cultivation of Desirable Traits: Ancient Indian farmers practiced mass selection, choosing plants with desirable traits for cultivation and seed saving.
Crop Diversity and Indigenous Varieties: India is known for its diverse crop varieties, many of which were developed through generations of selection by farmers.
Post-Independence Era (1947 onwards):
Formalization of Plant Breeding Programs: The Indian government established agricultural research institutes and universities, formalizing plant breeding efforts.
Introduction of Green Revolution Varieties: High-yielding varieties of wheat and rice were introduced, leading to a significant increase in food production.
Focus on Crop Improvement: Plant breeding programs targeted various crops like rice, wheat, millets, pulses, and oilseeds to improve yield, disease resistance, and other agronomic traits.
Recent Developments (21st Century):
Emphasis on Biotechnology and Molecular Breeding: Adoption of biotechnological tools like genetic engineering, marker-assisted breeding, and genomics for accelerated crop improvement.
Integration of Modern Techniques: Use of advanced technologies for trait introgression, hybrid development, and development of genetically modified (GM) crops.
Objectives of Plant Breeding:
Improving Yield and Productivity: Developing varieties with higher yield potential to meet the increasing global demand for food.
Enhancing Disease and Pest Resistance: Creating crops with genetic resistance to diseases and pests, reducing the reliance on chemical pesticides.
Adapting to Environmental Stresses: Breeding for tolerance to abiotic stresses like drought, salinity, and temperature extremes.
Methods for Creation of Variability:
Hybridization and Crossbreeding: Controlled pollination between different varieties or species to combine desirable traits.
Mutation Breeding: Inducing mutations through radiation or chemicals to create genetic variability.
Biotechnological Tools: Genetic engineering, CRISPR-Cas9 technology, and molecular markers for precise manipulation of genes.
Conclusion:
The history of plant breeding in India showcases a transition from traditional practices to modern scientific methods, with a continued focus on improving crop varieties for food security and sustainability.
(b) Describe Systemic Acquired Resistance (SAR) and source of disease resistance with suitable examples. Write the advantages of breeding for disease resistance in plants. (20 Marks)
Ans:
Introduction:
SAR is a defense mechanism in plants that provides long-lasting protection against a broad spectrum of pathogens.
Systemic Acquired Resistance (SAR):
Source of Disease Resistance:
Natural Genetic Variation:
Breeding for Resistance:
Advantages of Breeding for Disease Resistance:
Reduction in Chemical Inputs: Resistant varieties require fewer chemical pesticides, reducing environmental impact and production costs.
Sustainable Agriculture: Disease-resistant crops contribute to sustainable agriculture by reducing the reliance on chemical control measures.
Improved Yield Stability: Resistant varieties are less susceptible to yield losses caused by disease outbreaks, providing more stable crop yields.
Enhanced Food Security: Disease-resistant crops ensure a more reliable and consistent food supply, especially in regions prone to specific diseases.
Conclusion:
Breeding for disease resistance in plants is a vital component of sustainable agriculture, contributing to reduced environmental impact, increased yield stability, and improved food security.
(c) What do you understand by graft incompatibility ? Describe the symptoms and causes of graft incompatibility in plants with suitable examples. (10 Marks)
Ans:
Introduction:
Graft incompatibility is a condition where a graft union between two plant tissues fails to form a functional connection.
Symptoms of Graft Incompatibility:
Limited Growth or Vigor: The grafted scion may exhibit stunted growth or reduced overall vigor compared to a compatible graft.
Dieback or Wilt: The grafted scion may show symptoms of wilting, dieback of shoots, or general decline.
Callus Formation: Instead of a well-integrated union, a callus may form at the graft site, indicating a lack of successful union.
Causes of Graft Incompatibility:
Genetic Incompatibility: Differences in genetic makeup between the scion and rootstock can lead to incompatible grafts.
Physiological Mismatch: Differences in water, nutrient, or hormonal requirements between scion and rootstock can hinder successful grafting.
Disease or Pathogen Interaction: Presence of pathogens or diseases in one of the graft components can prevent successful union.
Examples of Graft Incompatibility:
Apple and Pear Grafting: Grafting apple (Malus domestica) onto pear (Pyrus spp.) rootstock often results in incompatibility due to genetic differences.
Citrus Grafting: Some citrus varieties exhibit incompatibility when grafted onto rootstocks of different species within the citrus genus.
Conclusion:
Understanding graft compatibility is crucial in successful horticultural practices. Incompatible grafts can lead to poor growth, reduced yields, and plant decline, highlighting the importance of selecting compatible plant materials for grafting projects.
Q4:
(a) Discuss different forms of Intellectual Property Rights (IPR) in India. (20 Marks)
Ans:
Introduction: Intellectual Property Rights are legal rights granted to individuals or organizations for their creations or inventions. In India, various forms of IPR are recognized and protected.
Forms of IPR in India:
1. Patents:
2. Copyright:
3. Trademarks:
4. Designs:
5. Geographical Indications (GI):
6. Plant Variety Protection (PVP):
Example: The Indian Patent Act of 1970 and subsequent amendments provide a framework for patent protection in India, ensuring inventors have the exclusive rights to their inventions for a specified period.
Conclusion:
India's recognition and protection of various forms of IPR play a crucial role in fostering innovation, creativity, and economic development.
(b) Give an account of stomate anatomy and cytology. Write down the effect of light, water deficit, CO2 concentration and temperature on stomatal movement along with the underlying mechanism. (20 Marks)
Ans:
Introduction:
Stomata are specialized pores on the surface of leaves that facilitate gas exchange. They consist of guard cells that regulate their opening and closing.
Stomatal Anatomy and Cytology:
1. Guard Cells:
2. Stomatal Pore:
Factors Affecting Stomatal Movement:
1. Light:
2. Water Deficit (Drought Stress):
3. CO2 Concentration:
4. Temperature:
Example: In a hot, dry environment, a plant will close its stomata to conserve water. This prevents excessive transpiration and helps the plant survive water stress.
Conclusion:
Understanding the factors influencing stomatal movement is crucial in agricultural and ecological contexts, as it directly impacts a plant's water-use efficiency and overall survival.
(c) Describe the role of State Seed Certification Agencies. (10 Marks)
Ans:
Introduction:
State Seed Certification Agencies play a vital role in ensuring the quality of seeds available to farmers, thereby enhancing agricultural productivity.
Functions of State Seed Certification Agencies:
Seed Testing and Quality Control: Conduct germination tests, purity tests, and other quality assessments to ensure seeds meet specified standards.
Certification of Seeds: Grant certification to seed lots that meet quality and genetic purity criteria, indicating they are suitable for planting.
Labeling and Tagging: Ensure that seed packages are properly labeled with essential information, including germination percentage, purity, and other details.
Field Inspection: Conduct inspections of seed production fields to verify adherence to isolation distances and other quality standards.
Varietal Purity Verification: Verify that seeds of registered varieties maintain their genetic purity through field inspections and laboratory tests.
Example: If a farmer purchases certified seeds for a specific crop, they can be assured that the seeds have undergone rigorous testing and meet quality standards, increasing the likelihood of a successful and productive crop.
Conclusion:
State Seed Certification Agencies play a critical role in maintaining the integrity and quality of seeds available to farmers, contributing to improved agricultural productivity and food security.
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