Membrane filter technique is a better test to identify Coliforms.

Explanation:

Coliforms are a group of bacteria commonly found in human and animal feces, as well as in soil and water. They are used as indicators of fecal contamination and the presence of pathogens. Therefore, it is important to identify and quantify the presence of coliforms in water and food samples. Several methods are available for the detection of coliforms, but the most suitable one is the membrane filter technique.

Membrane filter technique:

The membrane filter technique is a widely used method for the detection and enumeration of coliforms in water and food samples. The procedure involves filtering a known volume of sample through a membrane filter that retains the bacteria. The membrane is then placed onto a selective agar medium that promotes the growth of coliforms. After incubation, colonies of coliforms can be visually identified and counted, providing a quantitative measure of the coliform concentration in the sample.

Advantages of the membrane filter technique:

1. High sensitivity and specificity: The membrane filter technique is highly sensitive and specific for the detection of coliforms, with a detection limit of 1-10 CFU/100 mL.

2. Rapid results: The membrane filter technique provides rapid results, with detection and enumeration of coliforms within 24-48 hours.

3. Easy to perform: The membrane filter technique is easy to perform and requires minimal equipment and training.

4. Quantitative measure: The membrane filter technique provides a quantitative measure of the coliform concentration in the sample, which is useful for regulatory compliance and risk assessment.

Conclusion:

In conclusion, the membrane filter technique is a better test to identify coliforms due to its high sensitivity and specificity, rapid results, ease of use, and quantitative measure.