**Liquids Used in Preparing a Temporary Mount of a Leaf Peel to Observe Stomata**

In the experiment of preparing a temporary mount of a leaf peel to observe stomata, two liquids other than water are commonly used. These liquids are glycerine and iodine solution. They are used at different stages of the experiment to enhance the visibility of stomata and provide additional information about the structure and function of the leaf.

**Glycerine:**

- *When and Why Glycerine is used:*
Glycerine is used during the initial steps of the experiment. After obtaining a leaf peel, the first step is to place it on a glass slide. Glycerine is then applied to the leaf peel to replace the air present in the intercellular spaces of the leaf with the liquid. This is done by carefully adding a few drops of glycerine onto the leaf peel, ensuring that it completely covers the specimen.

- *Reason for using Glycerine:*
The primary reason for using glycerine is to prevent the leaf peel from drying out. Glycerine acts as a hydrating agent, keeping the cells of the leaf peel turgid and maintaining their structural integrity. This allows for a clearer observation of stomata under the microscope as the leaf peel remains plump and the stomata are not distorted due to dehydration.

**Iodine Solution:**

- *When and Why Iodine Solution is used:*
Iodine solution is used after the leaf peel has been hydrated with glycerine. A few drops of iodine solution are added to the glycerine-covered leaf peel. The iodine solution stains the cell contents, making them more visible under the microscope.

- *Reason for using Iodine Solution:*
Iodine solution contains iodine and potassium iodide, which react with starch present in the leaf cells. This reaction causes the starch to turn blue-black, highlighting its presence within the cells. By using iodine solution, we can determine the presence of starch in the leaf cells, which is an important indicator of photosynthetic activity. Additionally, the staining of cell contents also aids in distinguishing between different types of cells, such as guard cells and subsidiary cells, surrounding the stomata.

**Conclusion:**

In the experiment of preparing a temporary mount of a leaf peel to observe stomata, glycerine and iodine solution are used. Glycerine is used initially to keep the leaf peel turgid and prevent dehydration, while iodine solution is used to stain the cell contents and identify the presence of starch. Both liquids play crucial roles in enhancing the visibility of stomata and providing valuable information about the leaf's structure and function.

Safranin and glycerine are used while preparing temporary amount of peel to observe stomata other than water