A tube contained 20 ml of enzyme solution and the total activity of en...
1 Katal = 1 mol s-1
Specific activity = total enzyme activity per unit mass of protein
Total amount of protem in 20 ml of solution = 20 x 20 = 400 mg
Specific activity = 20/400 = 0.05 mol s-1 mg-1
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A tube contained 20 ml of enzyme solution and the total activity of en...
Calculating the specific enzyme activity
To calculate the specific enzyme activity, we need to divide the total activity of the enzyme by the protein concentration.
Given data:
- Enzyme solution volume = 20 ml
- Total activity of enzyme = 20 katal
- Protein concentration = 20 mg/ml
Step 1: Convert the enzyme activity to mol/s
The total activity of enzyme is given in katal. To convert it to mol/s, we need to use the definition of katal.
1 katal = 1 mol/s
Therefore, the total activity of enzyme in mol/s is also 20.
Step 2: Calculate the total amount of protein in the tube
To calculate the total amount of protein in the tube, we multiply the protein concentration by the volume of the enzyme solution.
Total amount of protein = Protein concentration x Enzyme solution volume
= 20 mg/ml x 20 ml
= 400 mg
Step 3: Convert the total amount of protein to grams
Since the specific enzyme activity is usually expressed in mol/s per gram of protein, we need to convert the total amount of protein to grams.
Total amount of protein in grams = Total amount of protein in mg / 1000
= 400 mg / 1000
= 0.4 g
Step 4: Calculate the specific enzyme activity
Finally, we can calculate the specific enzyme activity by dividing the total activity of enzyme by the total amount of protein.
Specific enzyme activity = Total activity of enzyme / Total amount of protein
= 20 mol/s / 0.4 g
= 50 mol/s/g
= 0.05 mol/s/mg (after converting grams to milligrams)
Therefore, the specific enzyme activity is 0.05 mol/s/mg.