Removal of RNA polymerase III from nucleoplasm will affect the synthesis of tRNA.


RNA polymerase III (Pol III) is an enzyme responsible for the transcription of various types of RNA molecules, including transfer RNA (tRNA), ribosomal RNA (rRNA), and small nuclear RNA (snRNA). It is one of the three RNA polymerases found in eukaryotic cells. Pol III is known for its role in transcribing genes that are involved in the translation machinery and essential cellular processes.


Transfer RNA (tRNA) is a type of RNA molecule that plays a crucial role in protein synthesis. It carries amino acids to the ribosome during translation, where they are added to the growing polypeptide chain. The synthesis of tRNA is essential for the proper functioning of protein synthesis machinery.


If RNA polymerase III is removed from the nucleoplasm, the synthesis of tRNA will be severely affected. This is because tRNA genes are transcribed by RNA polymerase III. Without the presence of this enzyme, the transcription of tRNA genes will be hindered or completely halted.


The removal of RNA polymerase III will have the following consequences on tRNA synthesis:

1. Reduced tRNA Levels: Without active RNA polymerase III, the transcription of tRNA genes will be impaired, leading to a decreased production of tRNA molecules. This will result in a shortage of tRNA available for protein synthesis.

2. Defective Protein Synthesis: Insufficient tRNA molecules will disrupt the accurate and efficient translation of mRNA into proteins. Each tRNA molecule carries a specific amino acid, and its absence or scarcity will limit the availability of amino acids for protein synthesis. This can lead to errors in the formation of the polypeptide chain and affect the overall protein synthesis process.

3. Cellular Dysfunction: Proteins are vital for carrying out cellular functions, and a deficiency in tRNA synthesis will impair the production of functional proteins. This can disrupt normal cellular processes and lead to cellular dysfunction or even cell death.

In conclusion, the removal of RNA polymerase III from the nucleoplasm will specifically impact the synthesis of tRNA. This will result in reduced tRNA levels, defective protein synthesis, and potential cellular dysfunction.