This is the standard curve for gel filtration chromatography. If any unknown protein is eluting out from gel filtration chromatogram in 35 min, the approx. molecular weight of that protein will be ______ [Answer upto one decimal place]Correct answer is between '17,18'. Can you explain this answer?

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This is the standard curve for gel filtration chromatography. If any unknown protein is eluting out from gel filtration chromatogram in 35 min, the approx. molecular weight of that protein will be ______ [Answer upto one decimal place]

Correct answer is between '17,18'. Can you explain this answer?

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This is the standard curve for gel filtration chromatography. If any u...

Thats why17.5 Kd will be molecular weight.

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Explanation:The separation techniques of proteins are used to isolate and purify proteins from complex mixtures. These techniques are based on various properties of proteins such as solubility, charge, size, and others. However, the technique of protein separation based on viscosity is not commonly used. Protein solubility:Protein solubility refers to the ability of a protein to dissolve in a given solvent. Different proteins have different solubilities in different solvents. This property is often used in techniques like precipitation, where proteins are selectively precipitated out of solution based on their solubility.Protein charge:Protein charge refers to the net charge of a protein molecule, which is determined by the presence of charged amino acid residues such as lysine, arginine, aspartic acid, and glutamic acid. Techniques like electrophoresis and ion exchange chromatography utilize the charge differences between proteins to separate them.Protein size:Protein size refers to the molecular weight or size of a protein molecule. Techniques such as gel filtration chromatography and SDS-PAGE separate proteins based on their size. In gel filtration chromatography, proteins are separated based on their ability to enter and pass through a porous gel matrix, while in SDS-PAGE, proteins are separated based on their migration through a gel under the influence of an electric field.Protein viscosity:Protein viscosity refers to the resistance of a protein solution to flow. While protein viscosity can vary depending on factors such as protein concentration, temperature, and pH, it is not typically used as a property for protein separation. In conclusion, the separation techniques of proteins are based on properties such as solubility, charge, and size. Protein viscosity is not commonly used as a property for protein separation.

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Which of the following methods can be used to determine the molecular weight of a given polypeptide.

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Protein was purified to homogeneity. Determination of the molecular weight by size exclusion chromatography yields 60 kD. Chromatography in the presence of 6 M urea yields a 30-kd species. When the chromatography is repeated in the presence of 6 M urea and 10 mM b-mercaptoethanol, a single molecular species of 15 kd results. Describe the structure of the molecule?

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Consider the reaction where one molecule of Z is disintegrating to form two molecules of X and one molecule of Y with rate k = 50 s-1. Suppose the reaction starts with 10000 molecules of Zat time t = 0. The average number of molecules of X at time t = 55 s will be ________. Correct answer is between '5400.0,5600.0'. Can you explain this answer?

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