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NEET Exam  >  NEET Questions  >  Living cells can be studied bya)phase contras... Start Learning for Free
Living cells can be studied by
  • a)
    phase contrast microscope
  • b)
    fluorescent microscope
  • c)
    electron microscope
  • d)
    light microscope
Correct answer is option 'A'. Can you explain this answer?
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Most Upvoted Answer
Living cells can be studied bya)phase contrast microscopeb)fluorescent...
Phase Contrast Microscope was developed by Zernike. The microscope has a phase plate and an annular diaphragm. They bring about changes in light rays passing through the specimen producing differences in light intensity. Denser parts alter path of light more than the thinner parts. This produces varying contrast for different regions. Hence, staining is not required and the difference between denser and lighter parts of the specimen is revealed by the phase difference. The microscope is useful in the study of small living organisms, living cells, constituents of living cells, effect of various factors over living structures, and study of various cellular events like behaviour of chromosomes during mitosis and meiosis, spindle formation, cytokinesis, pinocytosis, phagocytosis, cyclosis, glandular secretions, spermatogenesis, oogenesis etc.
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Read the following text and answer the following questions on the basis of the same:Electron Microscope Electron microscopes use electrons to illuminate a sample. In Transmission Electron Microscopy (TEM), electrons pass through the sample and illuminate film or a digital camera.Resolution in microscopy is limited to about half of the wavelength of the illumination source used to image the sample. Using visible light the best resolution that can be achieved by microscopes is about ~200 nm. Louis de Broglie showed that every particle or matter propagates like a wave. The wavelength of propagating electrons at a given accelerating voltage can be determined byThus, the wavelength of electrons is calculated to be 3.88 pm when the microscope is operated at 100 keV, 2. 74 pm at 200 keV and 2.24 pm at 300 keV. However, because the velocities of electrons in an electron microscope reach about 70% the speed of light with an accelerating voltage of 200 keV, there are relativistic effects on these electrons. Due to this effect, the wavelength at 100 keV, 200 keV and 300 keV in electron microscopes is 3.70 pm, 2.51 pm and 1.96 pm, respectively.Anyhow, the wavelength of electrons is much smaller than that of photons (2.5 pm at 200 keV). Thus if electron wave is used to illuminate the sample, the resolution of an electron microscope theoretically becomes unlimited. Practically, the resolution is limited to ~0.1 nm due to the objective lens system in electron microscopes. Thus, electron microscopy can resolve subcellular structures that could not be visualized using standard fluorescence microscopy.Q. Wavelength of electron as wave at accelerating voltage 200 keV is

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Living cells can be studied bya)phase contrast microscopeb)fluorescent microscopec)electron microscoped)light microscopeCorrect answer is option 'A'. Can you explain this answer?
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