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Electroporation procedure involves
- a)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.
- b)opening of stomatal pores during night by artificial light.
- c)making transient pores in the cell membrane to introduce gene constructs.
- d)purification of saline water with the help of a membrane system.
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
Electroporation procedure involvesa)fast passage of food through siev...
Electroporation is the method of making cell membrane permeable for the entry of recombinant DNA into the bacteria.
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Electroporation procedure involvesa)fast passage of food through siev...
Electroporation is a technique used in molecular biology to introduce genetic material, such as DNA or RNA, into cells. It involves the creation of transient pores in the cell membrane using an electrical pulse, allowing the genetic material to enter the cell. The correct answer is option C - making transient pores in the cell membrane to introduce gene constructs.
Explanation:
Electroporation is a widely used method for the introduction of nucleic acids into cells. It is a process that uses a brief electrical pulse to create temporary pores in the cell membrane, through which genetic material can enter the cell. This technique is commonly used in molecular biology research and has various applications, including gene transfection, gene expression analysis, and gene therapy.
The procedure involves the following steps:
1. Preparation of the cell suspension: The cells to be transfected are suspended in a suitable buffer or medium, which may contain the genetic material to be introduced.
2. Setting up the electroporation chamber: The cell suspension is placed in a specially designed electroporation chamber or cuvette. The chamber has two electrodes, between which the cells will be exposed to the electrical pulse.
3. Application of the electrical pulse: A short, high-voltage electrical pulse is applied to the cells. This pulse creates transient pores in the cell membrane, allowing the genetic material to enter the cells.
4. Recovery and analysis: After electroporation, the cells are allowed to recover in a suitable growth medium. The introduced genetic material can then be expressed and analyzed for its effects on the cells.
Key Points:
- Electroporation is a technique used to introduce genetic material into cells.
- The procedure involves the creation of transient pores in the cell membrane using an electrical pulse.
- This technique is commonly used in molecular biology research.
- It has various applications, including gene transfection, gene expression analysis, and gene therapy.
- The correct answer to the given question is option C - making transient pores in the cell membrane to introduce gene constructs.
Explanation:
Electroporation is a widely used method for the introduction of nucleic acids into cells. It is a process that uses a brief electrical pulse to create temporary pores in the cell membrane, through which genetic material can enter the cell. This technique is commonly used in molecular biology research and has various applications, including gene transfection, gene expression analysis, and gene therapy.
The procedure involves the following steps:
1. Preparation of the cell suspension: The cells to be transfected are suspended in a suitable buffer or medium, which may contain the genetic material to be introduced.
2. Setting up the electroporation chamber: The cell suspension is placed in a specially designed electroporation chamber or cuvette. The chamber has two electrodes, between which the cells will be exposed to the electrical pulse.
3. Application of the electrical pulse: A short, high-voltage electrical pulse is applied to the cells. This pulse creates transient pores in the cell membrane, allowing the genetic material to enter the cells.
4. Recovery and analysis: After electroporation, the cells are allowed to recover in a suitable growth medium. The introduced genetic material can then be expressed and analyzed for its effects on the cells.
Key Points:
- Electroporation is a technique used to introduce genetic material into cells.
- The procedure involves the creation of transient pores in the cell membrane using an electrical pulse.
- This technique is commonly used in molecular biology research.
- It has various applications, including gene transfection, gene expression analysis, and gene therapy.
- The correct answer to the given question is option C - making transient pores in the cell membrane to introduce gene constructs.
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Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer?
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Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer?.
Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer?.
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Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer?, a detailed solution for Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer? has been provided alongside types of Electroporation procedure involvesa)fast passage of food through sieve pores in phloem elements with the help of electric stimulation.b)opening of stomatal pores during night by artificial light.c)making transient pores in the cell membrane to introduce gene constructs.d)purification of saline water with the help of a membrane system.Correct answer is option 'C'. Can you explain this answer? theory, EduRev gives you an
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