Anaphase and Centromere Splitting:

Anaphase is the stage of cell division in which the sister chromatids are pulled apart towards opposite poles of the cell. This process is facilitated by the splitting of the centromere, a specialized region of a chromosome that holds the sister chromatids together.

Splitting of Centromere:
- When the centromere splits during anaphase, it results in the separation of the sister chromatids.
- Each chromatid is then considered a daughter chromosome.

Formation of Daughter Chromosomes:
- The splitting of the centromere results in the formation of daughter chromosomes.
- These daughter chromosomes are non-identical because they contain different genetic material from the parent cell.
- Each daughter chromosome will have a unique combination of genes that may differ from the original chromosome.

Genetic Identity of Daughter Nuclei:
- After the splitting of the centromere and the separation of sister chromatids, two daughter nuclei are formed.
- These daughter nuclei will contain genetically identical chromosomes.
- Each daughter nucleus will have a complete set of chromosomes that is identical to the parent cell.

Unequal Division of Genetic Material:
- During anaphase, the splitting of the centromere ensures that each daughter cell receives an equal and identical set of chromosomes.
- This process helps maintain genetic stability and ensures that each daughter cell has the correct number of chromosomes.
- Therefore, there is no unequal division of genetic material during anaphase and the splitting of the centromere.

In conclusion, the splitting of the centromere during anaphase results in the separation of sister chromatids, the formation of non-identical daughter chromosomes, and the creation of genetically identical daughter nuclei. This process ensures the accurate division of genetic material and the inheritance of correct genetic information by each daughter cell.