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During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
  • a)
    Annealing
  • b)
    Extension
  • c)
    Denaturation
  • d)
    Ligation
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
During the process of gene amplification using PCR, if very high temp...
  • Option (c) is correct. High temperature about 94°C is required for the process of denaturation which is the first step of PCR.
  • Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase.
  • Annealing is performed at 50°-60°C which is the second step that can get affected.
  • Addition of nucleotides to the primer, synthesizing a new DNA strand using only the template sequences with the help of enzyme DNA polymerase is called primer extension/ polymerisation.
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During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?a)Annealingb)Extensionc)Denaturationd)LigationCorrect answer is option 'C'. Can you explain this answer?
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