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Are there any specific plant biotechnology techniques or applications that I should be familiar with for the UPSC Botany optional subject exam?
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Plant Biotechnology Techniques and Applications for UPSC Botany Optional Exam


1. Genetic Engineering


  • Recombinant DNA Technology: The process of combining DNA molecules from different sources to create a new genetic combination.

  • Gene Cloning: The replication and production of multiple copies of a specific DNA segment.

  • Transgenic Plants: Plants that have been genetically modified by introducing foreign genes into their genomes.

  • Marker-Assisted Selection: The use of molecular markers to identify and select plants with desirable traits.



2. Plant Tissue Culture


  • Micropropagation: The rapid multiplication of plants through the culture of small plant parts in a sterile environment.

  • Somatic Embryogenesis: The development of embryos from somatic cells without the involvement of sexual reproduction.

  • Protoplast Culture: The isolation and cultivation of plant cells without cell walls.

  • Embryo Rescue: The technique of rescuing embryos from incompatible crosses and growing them in vitro.



3. Molecular Markers


  • RFLP (Restriction Fragment Length Polymorphism): A technique for analyzing DNA variations by cutting DNA with restriction enzymes and separating the resulting fragments.

  • SSR (Simple Sequence Repeat): Repeated DNA sequences used as markers to identify genetic variations.

  • SNP (Single Nucleotide Polymorphism): A variation at a single base pair in DNA.

  • AFLP (Amplified Fragment Length Polymorphism): A PCR-based technique used to detect DNA polymorphism.



4. Molecular Breeding


  • Marker-Assisted Selection (MAS): The use of molecular markers to identify and select plants with desirable traits.

  • Genome Editing: Techniques like CRISPR-Cas9 are used to modify specific genes in plant genomes.

  • Gene Silencing: Techniques like RNA interference (RNAi) are used to suppress the expression of specific genes.

  • Transcriptomics and Proteomics: The study of gene expression and protein profiles to understand plant functions and traits.



5. Plant Transformation


  • Agrobacterium-Mediated Transformation: The use of Agrobacterium tumefaciens to transfer genes into plant cells.

  • Particle Bombardment: The use of gene guns to deliver DNA-coated particles into plant cells.

  • Electroporation: The use of electric pulses to create temporary pores in plant cell membranes for DNA uptake.



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Are there any specific plant biotechnology techniques or applications that I should be familiar with for the UPSC Botany optional subject exam?
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