Introduction:
DNA barcoding is a technique used to identify and classify organisms based on their DNA sequences. It involves analyzing a specific region of an organism's DNA to create a barcode-like pattern that can be used for identification purposes. The main application of DNA barcoding mentioned in the text is the identification of plant leaves.

Identification of plant leaves:
DNA barcoding has been widely used in the identification of plant species based on their leaves. Each plant species has a unique DNA sequence that can be used to distinguish it from other species. By comparing the DNA barcode of an unknown plant leaf with a reference database of known plant species, scientists can quickly and accurately identify the species to which it belongs.

Advantages of DNA barcoding in identifying plant leaves:
1. Accuracy: DNA barcoding provides a highly accurate method for identifying plant species. It eliminates the potential for human error that can occur with traditional methods of identification, such as relying on morphological characteristics.

2. Speed: DNA barcoding is a rapid technique that can provide results within a short period of time. This is particularly useful when dealing with large numbers of plant samples that need to be identified.

3. Non-destructive: DNA barcoding can be performed using small leaf samples, which do not require the destruction of the entire plant. This is important for conserving rare or endangered plant species.

4. Overcoming limitations of traditional methods: Traditional methods of plant identification, such as relying on physical characteristics or microscopic examination, can be challenging, especially when dealing with dried or processed plant materials. DNA barcoding can overcome these limitations by providing a molecular-based identification method.

Conclusion:
In conclusion, the main application of DNA barcoding mentioned in the text is the identification of plant leaves. DNA barcoding offers several advantages in this context, including accuracy, speed, non-destructiveness, and the ability to overcome limitations of traditional identification methods. This technique has revolutionized the field of plant taxonomy and has become an essential tool for plant identification and classification.

DNA bar-coding is primarily used for identifying plant leaves, even in the absence of fruit. It is a valuable tool for species identification in various fields, including botany.