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Which one of the following statements best describes the purpose of 'Site Directed Nuclease (SDN)'?
- a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.
- b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.
- c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.
- d)It is a technology to efficiently extract nuclear fuel from the uranium ores.
Correct answer is option 'B'. Can you explain this answer?
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Which one of the following statements best describes the purpose of Si...
- SDN or sequence specific nuclease (SSN) refers to the practice of cleaving DNA strands to affect the subsequent genome editing. SDN technology takes advantage of targeted DNA break and host’s natural repair mechanisms to introduce specific small changes at the site of the DNA break.
- The basis of current targeted genome editing applications is the capacity to induce a DNA double strand break (DSB) at a selected location in the genome where the modification is intended. Directed repair of the DSB allows for targeted genome editing. Such applications can be applied to generate mutations (targeted mutations or precise native gene editing) as well as precise insertion of genes (cisgenes, intragenes, or transgenes).
- Different approaches can be used to achieve targeted DNA breaks, including Meganucleases (MN), Zinc Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs) and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated proteins (CRISPR/Cas) etc. Collectively, these are often discussed under the acronym site directed nucleases (SDNs), pointing out to the general principle of the technology to use a DNA cutting enzyme (nuclease) for the generation of the targeted (or site directed) DNA break. Hence option (b) is the correct answer.
- Variants of SDN applications are often categorized as SDN-1, SDN-2 and SDN-3 depending on the outcome of the DNA double strand break repair.
- SDN-1: When the SDN is used in the absence of a DNA repair template (see SDN-2/-3), the outcome is a targeted, non-specific genetic deletion mutation. In this case, the position of the DNA DSB is precisely selected, but the DNA repair by the host cell is random and results in small nucleotide deletions, additions or substitutions.
- SDN-2: Is used to generate gene editing mutations. In this case, a SDN is used to generate a targeted DSB and a DNA repair template (a short DNA sequence identical to the targeted DSB DNA sequence except for one or a few nucleotide changes) is used to repair the DSB. The outcome is a targeted and predetermined point mutation in the desired gene of interest.
- SDN-3: When the SDN is used along with a DNA repair template that contains new DNA sequence (e.g.gene), the outcome of the technology would be the integration of that DNA sequence into the plant genome. The most likely application illustrating the use of SDN-3 would be the insertion of cisgenic, intragenic, or transgenic expression cassettes at a selected genome location.
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Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer?
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Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer? for UPSC 2024 is part of UPSC preparation. The Question and answers have been prepared according to the UPSC exam syllabus. Information about Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for UPSC 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer?.
Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer? for UPSC 2024 is part of UPSC preparation. The Question and answers have been prepared according to the UPSC exam syllabus. Information about Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for UPSC 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer?.
Solutions for Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer? in English & in Hindi are available as part of our courses for UPSC.
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ample number of questions to practice Which one of the following statements best describes the purpose of Site Directed Nuclease (SDN)?a)It represents a mitochondrial replacement therapy where male and female pronuclei are removed from each fertilized egg prior to their fusing.b)It denotes the DNA cutting enzymes used for the generation of the targeted DNA breaks.c)It represents the latest eco-friendly technology developed for the remediation of oil sludge and oil spills.d)It is a technology to efficiently extract nuclear fuel from the uranium ores.Correct answer is option 'B'. Can you explain this answer? tests, examples and also practice UPSC tests.
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