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SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings:
A. Transcription of gene X is coupled to mRNA capping
B. Transcription elongation is coupled to splicing.
C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes
D. Both capping and splicing of mRNA occur simultaneously.
Identify the correct set of conclusions
- a)A B and C
- b)B C and D
- c)A C and D
- d)A B and D
Correct answer is option 'A'. Can you explain this answer?
Verified Answer
SiHa cell extract was used to study the transcription of gene X, which...
Concept:
- Transcription is divided into three major stages, namely, initiation, chain elongation and chain termination.
- Initiation consists of RNAP binding to a specific promoter DNA and strand separation of the DNA that allows NTP binding to the template strand at the enzyme active site.
- Elongation is a repetitive but temporally discontinuous formation of phosphodiester bonds.
- Termination describes the complete dissociation of the RNA strand from the RNA-DNA hybrid retained in RNAP in response to specific signals.
- Different from initiation or termination, which occurs at specific DNA sites, elongation involves a gene-scale DNA tracking system, accompanied by bond formation at every base pair.
- Hence, specific DNA conformations/flexibilities, DNA lesions, and DNA-binding proteins are of specific concern in the regulation of elongation.
Explanation:
- In eukaryotes is functionally coupled to mRNA maturation which includes capping, splicing, and polyadenylation. Alternative splicing and transcription elongation by RNA polymerase II (RNAPII) are two processes which are tightly connected.
- Splicing is a co-transcriptional process, and different experimental approaches show that splicing is coupled to transcription in Drosophila, yeast, and mammals the process of splicing coincides with intron exit from RNAPII: with high-resolution sequencing techniques, nascent RNAs from 87 yeast genes were analyzed (about one third of intron containing genes in this organism).
- The recruitment coupling focuses on recruitment of splicing factors by the transcription machinery: RNAPII largest subunit contains a carboxy-terminal domain (CTD).
- In animals and plants, the CTD is composed of a number of repeats of a consensus heptad YSPTSPST that can be subject to different post-translational modifications in their residues.
- Modifications of the CTD regulate the affinity for factors involved in capping, 3′ end processing and alternative splicing.
- The CTD phosphorylation patterns determinate the recruitment of splicing factors to transcription sites.
Statement A:
Transcription of gene X is coupled to mRNA capping
- Consider the explanation above thus this option is true, multiple evidences for coupling between transcription and alternative splicing have been obtained working with mammalian culture cells
Statement B: T
ranscription elongation is coupled to splicing.
- Consider the explanation above thus this option is true ,multiple evidences for coupling between transcription and alternative splicing have been obtained working with mammalian culture cells
Statement C:
Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzyme
- Consider the explanation above thus this option is true, as modifications of the CTD regulate the affinity for factors involved in capping, 3′ end processing and alternative splicing
Statement D:
Both capping and splicing of mRNA occur simultaneously.
- Consider the explanation above thus this option is not true.
hence the correct answer is option 3
Most Upvoted Answer
SiHa cell extract was used to study the transcription of gene X, which...
Explanation:
Transcription of gene X is coupled to mRNA capping:
- The presence of both capping and splicing enzymes in the RNA Pol II complex indicates that mRNA capping is coupled to transcription of gene X.
- This suggests that capping occurs during the transcription process, ensuring that the mRNA is properly modified before it is fully synthesized.
Transcription elongation is coupled to splicing:
- When transcription elongation was inhibited, the complex contained only capping enzymes, indicating that splicing enzymes are associated with the elongating polymerase complex.
- This suggests that splicing occurs during transcription elongation, potentially allowing for efficient processing of the pre-mRNA.
Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes:
- When the phosphorylation of the CTD domain of Pol II was inhibited, the complex contained neither splicing nor capping enzymes.
- This indicates that phosphorylation of the CTD domain is necessary for the recruitment of both capping and splicing enzymes to the polymerase complex.
- This phosphorylation event may serve as a critical regulatory step in coordinating transcription, capping, and splicing processes.
Therefore, the correct set of conclusions from the findings is A. Transcription of gene X is coupled to mRNA capping.
Transcription of gene X is coupled to mRNA capping:
- The presence of both capping and splicing enzymes in the RNA Pol II complex indicates that mRNA capping is coupled to transcription of gene X.
- This suggests that capping occurs during the transcription process, ensuring that the mRNA is properly modified before it is fully synthesized.
Transcription elongation is coupled to splicing:
- When transcription elongation was inhibited, the complex contained only capping enzymes, indicating that splicing enzymes are associated with the elongating polymerase complex.
- This suggests that splicing occurs during transcription elongation, potentially allowing for efficient processing of the pre-mRNA.
Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes:
- When the phosphorylation of the CTD domain of Pol II was inhibited, the complex contained neither splicing nor capping enzymes.
- This indicates that phosphorylation of the CTD domain is necessary for the recruitment of both capping and splicing enzymes to the polymerase complex.
- This phosphorylation event may serve as a critical regulatory step in coordinating transcription, capping, and splicing processes.
Therefore, the correct set of conclusions from the findings is A. Transcription of gene X is coupled to mRNA capping.
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SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer?
Question Description
SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer?.
SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer?.
Solutions for SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? in English & in Hindi are available as part of our courses for Software Development.
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Here you can find the meaning of SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of
SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer?, a detailed solution for SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? has been provided alongside types of SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice SiHa cell extract was used to study the transcription of gene X, which has six introns. The RNA Pol II complex containing all associated proteins was isolated from the actively transcribing system and subjected to proteome analysis. Results showed the presence of both splicing and capping enzymes in the complex. When transcription elongation was inhibited by flavopiridol, the polymerase complex contained only capping enzymes. When phosphorylation of the CTD domain of Pol II was inhibited by a kinase inhibitor, the complex contained neither splicing nor capping enzymes. The following conclusions were drawn from these findings: A. Transcription of gene X is coupled to mRNA capping B. Transcription elongation is coupled to splicing. C. Phosphorylation of CTD domain is required for the recruitment of capping and splicing enzymes D. Both capping and splicing of mRNA occur simultaneously. Identify the correct set of conclusions a)A B and Cb)B C and Dc)A C and Dd)A B and DCorrect answer is option 'A'. Can you explain this answer? tests, examples and also practice Software Development tests.
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