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In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.
- a)No difference from intact DNA pol I replication
- b)Replication will be slower
- c)Replication will be error-prone
- d)DNA produced will be shorter
Correct answer is option 'D'. Can you explain this answer?
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In an experiment, you use the DNA pol I – Klenow fragment. When ...
In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, the DNA produced will be shorter.
Klenow fragment provides the 5’ -> 3’ exonuclease activity used to remove the primer. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini. In its absence, the primer remains and hence DNA produced is shorter (i.e. original length-length of primer).
Klenow fragment provides the 5’ -> 3’ exonuclease activity used to remove the primer. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini. In its absence, the primer remains and hence DNA produced is shorter (i.e. original length-length of primer).
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In an experiment, you use the DNA pol I – Klenow fragment. When ...
Effect of using DNA pol I – Klenow fragment on leading strand replication:
Shorter DNA production:
When using DNA pol I – Klenow fragment for leading strand replication, the newly replicated DNA will be shorter than intact DNA pol I replication. This is because the Klenow fragment lacks the 5' to 3' exonuclease activity needed for proofreading and correcting errors during replication. As a result, mistakes in base pairing are more likely to occur, leading to shorter DNA fragments being produced.
Implications of shorter DNA production:
1. Shorter DNA fragments may result in incomplete genes or non-functional proteins being synthesized.
2. Errors in replication could lead to mutations in the DNA sequence, potentially causing genetic disorders or diseases.
3. The lack of proofreading ability increases the chances of introducing errors in the DNA sequence, affecting the accuracy of genetic information transmission.
Conclusion:
In conclusion, using the DNA pol I – Klenow fragment for leading strand replication can result in shorter DNA production due to the absence of proofreading activity. This can have significant implications for the accuracy and functionality of the replicated DNA strand.
Shorter DNA production:
When using DNA pol I – Klenow fragment for leading strand replication, the newly replicated DNA will be shorter than intact DNA pol I replication. This is because the Klenow fragment lacks the 5' to 3' exonuclease activity needed for proofreading and correcting errors during replication. As a result, mistakes in base pairing are more likely to occur, leading to shorter DNA fragments being produced.
Implications of shorter DNA production:
1. Shorter DNA fragments may result in incomplete genes or non-functional proteins being synthesized.
2. Errors in replication could lead to mutations in the DNA sequence, potentially causing genetic disorders or diseases.
3. The lack of proofreading ability increases the chances of introducing errors in the DNA sequence, affecting the accuracy of genetic information transmission.
Conclusion:
In conclusion, using the DNA pol I – Klenow fragment for leading strand replication can result in shorter DNA production due to the absence of proofreading activity. This can have significant implications for the accuracy and functionality of the replicated DNA strand.
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In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer?
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In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer?.
In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer?.
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In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer?, a detailed solution for In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer? has been provided alongside types of In an experiment, you use the DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.a)No difference from intact DNA pol I replicationb)Replication will be slowerc)Replication will be error-proned)DNA produced will be shorterCorrect answer is option 'D'. Can you explain this answer? theory, EduRev gives you an
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