Translocation of tRNA from P site to E site in bacteria
Translocation of tRNA from the P site to the E site during translation elongation in bacteria is an essential step in protein synthesis. This process requires the hydrolysis of GTP molecules to provide energy for the movement of the ribosome along the mRNA.

Number of GTP molecules required
- During the translocation process, two GTP molecules are required for the movement of the ribosome and the tRNA from the P site to the E site.
- The first GTP molecule is used to bind the elongation factor G (EF-G) to the ribosome.
- The second GTP molecule is hydrolyzed by EF-G, providing the energy needed for the translocation of the ribosome and the tRNA.

Role of GTP in translocation
- GTP is a high-energy molecule that serves as a source of energy for various cellular processes.
- In the context of translation elongation, GTP hydrolysis provides the energy required for the movement of the ribosome along the mRNA and the translocation of tRNA from one site to another.
- Without the hydrolysis of GTP, the ribosome would not be able to move along the mRNA and synthesize proteins effectively.
In conclusion, two GTP molecules are required for the translocation of tRNA from the P site to the E site during translation elongation in bacteria. This process is essential for the accurate and efficient synthesis of proteins in bacterial cells.