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Consider the following statements regarding the CRISPR-Cas9 system and the FnCas9 enzyme:
1. FnCas9 is derived from the bacterium Francisella novicida and is known for its higher specificity in targeting DNA sequences compared to SpCas9.
2. The CRISPR-Cas9 system uses a specifically designed RNA molecule known as guide RNA (gRNA) to cut DNA at a location specified by the gRNA.
3. Proto-spacer Adjacent Motif (PAM) is a long DNA sequence adjacent to the target site that Cas9 must recognize and bind to in order to cut the DNA.
Which of the statements given above is/are correct?
  • a)
    1 Only
  • b)
    1 and 2 Only
  • c)
    1 and 3 Only
  • d)
    1, 2, and 3
Correct answer is option 'B'. Can you explain this answer?
Verified Answer
Consider the following statements regarding the CRISPR-Cas9 system an...
Statement 1 is correct. FnCas9 is indeed derived from the bacterium Francisella novicida. It is noted for its higher specificity in targeting DNA sequences compared to SpCas9, which helps in reducing off-target effects and ensures more reliable genome editing.
Statement 2 is also correct. The CRISPR-Cas9 system uses a guide RNA (gRNA), which is a specifically designed RNA molecule that matches the target DNA sequence. This gRNA directs the Cas9 enzyme to the correct location on the DNA, acting as molecular scissors that cut the DNA at the specified location.
Statement 3 is incorrect. The Proto-spacer Adjacent Motif (PAM) is actually a short DNA sequence adjacent to the target site that the Cas9 enzyme must recognize and bind to in order to cut the DNA, not a long sequence. This PAM sequence is crucial for the binding and activity of the Cas9 enzyme.
Therefore, the correct statements are 1 and 2 only.
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Consider the following statements regarding the CRISPR-Cas9 system and the FnCas9 enzyme:1. FnCas9 is derived from the bacterium Francisella novicida and is known for its higher specificity in targeting DNA sequences compared to SpCas9.2. The CRISPR-Cas9 system uses a specifically designed RNA molecule known as guide RNA (gRNA) to cut DNA at a location specified by the gRNA.3. Proto-spacer Adjacent Motif (PAM) is a long DNA sequence adjacent to the target site that Cas9 must recognize and bind to in order to cut the DNA.Which of the statements given above is/are correct?a)1 Onlyb)1 and 2 Onlyc)1 and 3 Onlyd)1, 2, and 3Correct answer is option 'B'. Can you explain this answer?
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Consider the following statements regarding the CRISPR-Cas9 system and the FnCas9 enzyme:1. FnCas9 is derived from the bacterium Francisella novicida and is known for its higher specificity in targeting DNA sequences compared to SpCas9.2. The CRISPR-Cas9 system uses a specifically designed RNA molecule known as guide RNA (gRNA) to cut DNA at a location specified by the gRNA.3. Proto-spacer Adjacent Motif (PAM) is a long DNA sequence adjacent to the target site that Cas9 must recognize and bind to in order to cut the DNA.Which of the statements given above is/are correct?a)1 Onlyb)1 and 2 Onlyc)1 and 3 Onlyd)1, 2, and 3Correct answer is option 'B'. Can you explain this answer? for UPSC 2025 is part of UPSC preparation. The Question and answers have been prepared according to the UPSC exam syllabus. Information about Consider the following statements regarding the CRISPR-Cas9 system and the FnCas9 enzyme:1. FnCas9 is derived from the bacterium Francisella novicida and is known for its higher specificity in targeting DNA sequences compared to SpCas9.2. The CRISPR-Cas9 system uses a specifically designed RNA molecule known as guide RNA (gRNA) to cut DNA at a location specified by the gRNA.3. Proto-spacer Adjacent Motif (PAM) is a long DNA sequence adjacent to the target site that Cas9 must recognize and bind to in order to cut the DNA.Which of the statements given above is/are correct?a)1 Onlyb)1 and 2 Onlyc)1 and 3 Onlyd)1, 2, and 3Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for UPSC 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Consider the following statements regarding the CRISPR-Cas9 system and the FnCas9 enzyme:1. FnCas9 is derived from the bacterium Francisella novicida and is known for its higher specificity in targeting DNA sequences compared to SpCas9.2. The CRISPR-Cas9 system uses a specifically designed RNA molecule known as guide RNA (gRNA) to cut DNA at a location specified by the gRNA.3. Proto-spacer Adjacent Motif (PAM) is a long DNA sequence adjacent to the target site that Cas9 must recognize and bind to in order to cut the DNA.Which of the statements given above is/are correct?a)1 Onlyb)1 and 2 Onlyc)1 and 3 Onlyd)1, 2, and 3Correct answer is option 'B'. Can you explain this answer?.
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