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Using a single template molecule, how many DNA molecules are generated after 10 cycles of Amplification in PCR. class 12 biology.?
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Using a single template molecule, how many DNA molecules are generated...
Understanding PCR Amplification
Polymerase Chain Reaction (PCR) is a widely used technique in molecular biology that allows for the amplification of a specific DNA segment. Each cycle of PCR doubles the amount of DNA present, leading to exponential growth.
Initial Conditions
- Starting with 1 template DNA molecule.
- Each cycle involves denaturation, annealing, and extension.
Cycle Breakdown
1. Cycle 1:
- 1 template → 2 molecules
2. Cycle 2:
- 2 molecules → 4 molecules
3. Cycle 3:
- 4 molecules → 8 molecules
4. Cycle 4:
- 8 molecules → 16 molecules
5. Cycle 5:
- 16 molecules → 32 molecules
6. Cycle 6:
- 32 molecules → 64 molecules
7. Cycle 7:
- 64 molecules → 128 molecules
8. Cycle 8:
- 128 molecules → 256 molecules
9. Cycle 9:
- 256 molecules → 512 molecules
10. Cycle 10:
- 512 molecules → 1024 molecules
Final Count
- After 10 cycles, the total number of DNA molecules generated is 1024.
Exponential Growth Formula
- The formula to calculate the number of DNA molecules after n cycles is:
- Total DNA = 2^n
- For n = 10, Total DNA = 2^10 = 1024 molecules.
Conclusion
PCR is a powerful tool that efficiently amplifies DNA, making it essential for various applications in research, forensics, and medical diagnostics. After 10 cycles, starting from a single template molecule, PCR yields 1024 DNA molecules.
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Using a single template molecule, how many DNA molecules are generated after 10 cycles of Amplification in PCR. class 12 biology.?
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