The biosphere is made up of ( Hydrosphere which means water , lithosphere which means land, Atmosphere which means air) so we simply conclude that the First one is not the part of biosphere which is Asthenosphere.

Explanation:
The biosphere is the zone of life on Earth, where living organisms exist. It includes all the ecosystems and habitats on the planet. The biosphere is composed of several interconnected spheres, each of which plays a vital role in supporting life on Earth. These spheres include the atmosphere, hydrosphere, lithosphere, and biosphere itself.

Atmosphere:
The atmosphere is the layer of gases that surrounds the Earth. It consists of nitrogen, oxygen, carbon dioxide, and other trace gases. It provides the air we breathe and protects us from harmful radiation from the sun. The atmosphere is an integral part of the biosphere as it contains the necessary gases for respiration and photosynthesis.

Hydrosphere:
The hydrosphere refers to all the water on Earth, including oceans, lakes, rivers, groundwater, and even water vapor in the atmosphere. It is essential for the survival of living organisms as it provides a habitat for aquatic life and is involved in various ecological processes such as the water cycle.

Lithosphere:
The lithosphere is the solid outer layer of the Earth. It includes the Earth's crust and the uppermost part of the mantle. The lithosphere provides a physical substrate for life, supporting the growth of plants and serving as habitats for various organisms. It also contains valuable resources that humans depend on, such as minerals and fossil fuels.

Asthenosphere:
The asthenosphere is a layer of the Earth's mantle located beneath the lithosphere. It is a semi-fluid layer composed of hot, partially molten rock. The asthenosphere plays a crucial role in plate tectonics, where the movement of tectonic plates occurs. While it is an important component of the Earth's geology, it does not directly interact with the biosphere.

Conclusion:
In conclusion, the asthenosphere does not make up the biosphere. The biosphere is composed of the atmosphere, hydrosphere, lithosphere, and the life forms within it. The asthenosphere, although a significant component of the Earth's structure, does not directly interact with the biosphere.