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Biotechnology - Principle & Processes PPT Biology Class 12

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PRINCIPLES AND PROCESSES
Page 2


PRINCIPLES AND PROCESSES
BIOTECHNOLOGY: PRINCIPLES
AND PROCESSES
Biotechnology deals with techniques of using live 
organisms or enzymes from organisms to produce products 
and processes useful to humans.
The European Federation of Biotechnology (EFB) has given a
definition of biotechnology that encompasses both traditional
view and modern molecular biotechnology.
The definition given by EFB is as follows:
 
‘The integration of natural science and organisms, cells, parts
thereof, and molecular analogues for products and services’.
Page 3


PRINCIPLES AND PROCESSES
BIOTECHNOLOGY: PRINCIPLES
AND PROCESSES
Biotechnology deals with techniques of using live 
organisms or enzymes from organisms to produce products 
and processes useful to humans.
The European Federation of Biotechnology (EFB) has given a
definition of biotechnology that encompasses both traditional
view and modern molecular biotechnology.
The definition given by EFB is as follows:
 
‘The integration of natural science and organisms, cells, parts
thereof, and molecular analogues for products and services’.
Asexual reproduction preserves the genetic identity of
species.
Sexual reproduction creates variation and creates unique
combinations of genetic makeup.
Traditional hybridization procedures used in plant and
animal breeding lead to inclusion of undesired genes with
desired genes
The techniques of genetic engineering which includes
creation of recombinant DNA, use of gene cloning and
gene transfer, help to overcome this limitation  
 Principle of genetic
engineering
Page 4


PRINCIPLES AND PROCESSES
BIOTECHNOLOGY: PRINCIPLES
AND PROCESSES
Biotechnology deals with techniques of using live 
organisms or enzymes from organisms to produce products 
and processes useful to humans.
The European Federation of Biotechnology (EFB) has given a
definition of biotechnology that encompasses both traditional
view and modern molecular biotechnology.
The definition given by EFB is as follows:
 
‘The integration of natural science and organisms, cells, parts
thereof, and molecular analogues for products and services’.
Asexual reproduction preserves the genetic identity of
species.
Sexual reproduction creates variation and creates unique
combinations of genetic makeup.
Traditional hybridization procedures used in plant and
animal breeding lead to inclusion of undesired genes with
desired genes
The techniques of genetic engineering which includes
creation of recombinant DNA, use of gene cloning and
gene transfer, help to overcome this limitation  
 Principle of genetic
engineering
Three basic steps in genetically modifying an
organism–
Identification of DNA with desirable gene
Introduction of the identified DNA into the host.
 Maintenance of introduced DNA in the host and
transfer ofthe DNA to its progeny.
Page 5


PRINCIPLES AND PROCESSES
BIOTECHNOLOGY: PRINCIPLES
AND PROCESSES
Biotechnology deals with techniques of using live 
organisms or enzymes from organisms to produce products 
and processes useful to humans.
The European Federation of Biotechnology (EFB) has given a
definition of biotechnology that encompasses both traditional
view and modern molecular biotechnology.
The definition given by EFB is as follows:
 
‘The integration of natural science and organisms, cells, parts
thereof, and molecular analogues for products and services’.
Asexual reproduction preserves the genetic identity of
species.
Sexual reproduction creates variation and creates unique
combinations of genetic makeup.
Traditional hybridization procedures used in plant and
animal breeding lead to inclusion of undesired genes with
desired genes
The techniques of genetic engineering which includes
creation of recombinant DNA, use of gene cloning and
gene transfer, help to overcome this limitation  
 Principle of genetic
engineering
Three basic steps in genetically modifying an
organism–
Identification of DNA with desirable gene
Introduction of the identified DNA into the host.
 Maintenance of introduced DNA in the host and
transfer ofthe DNA to its progeny.
Restriction Enzymes
 Cloning vectors 
Competent Host (for transformation with
recombinant DNA)
TOOLS OF RECOMBINANT
DNA TECHNOLOGY
Genetic engineering or recombinant DNA technology can be
accomplished only if we have the key tools -
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FAQs on Biotechnology - Principle & Processes PPT Biology Class 12

1. What are the principles of biotechnology?
Ans. The principles of biotechnology involve the use of biological systems, organisms, or derivatives to develop or modify products, processes, or living organisms for specific purposes. It includes techniques such as genetic engineering, fermentation, and tissue culture.
2. What are the processes involved in biotechnology?
Ans. Biotechnology processes include DNA extraction, gene cloning, PCR (Polymerase Chain Reaction), DNA sequencing, protein expression, and purification. These processes are used to manipulate and study DNA, genes, and proteins for various applications.
3. How is genetic engineering used in biotechnology?
Ans. Genetic engineering is a technique used in biotechnology to modify an organism's genetic material by introducing specific genes or altering existing ones. It allows scientists to create genetically modified organisms (GMOs) with desired traits, such as increased crop yield or disease resistance.
4. What is fermentation in biotechnology?
Ans. Fermentation is a biotechnology process that involves the conversion of sugars into alcohol or organic acids using microorganisms. It is commonly used in the production of alcoholic beverages, biofuels, and various industrial chemicals.
5. What is the significance of tissue culture in biotechnology?
Ans. Tissue culture is an important technique in biotechnology that involves the growth and maintenance of plant or animal cells in a controlled environment. It is used for the production of disease-free plants, plant breeding, and the study of cell behavior and development. Tissue culture also plays a crucial role in the production of pharmaceuticals and the preservation of endangered species.
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