Q.1. The number of substrate level phosphorylations in one turn of citric acid cycle is : (2020)
During Krebs' or citric acid cycle, succinyl-CoA is acted upon by enzyme succinyl-CoA synthetase to form succinate (a 4C compound). The reaction releases sufficient energy to form ATP (in plants) or GTP (in animals) by substrate-level phosphorylation. GTP can then be used to form ATP.
Q.2. Conversion of glucose to glucose-6-phosphate, the first irreversible reaction of glycolysis, is catalysed by (2019)
Glucose is phosphorylated to glucose-6- phosphate by ATP in presence of enzyme hexokinase and Mg2+.
Q.3. Respiratory Quotient (RQ) value of tripalmitin is (2019)
Respiratory Quotient (RQ) is the ratio of the volume of carbon dioxide produced to the volume of oxygen consumed in respiration over a period of time.
RQ for fats is about 0.7.
Tripalmitin = C51H98O6
2(C51H98O6) + 145 O2 → 102 CO2 + 98 H2O
RQ = 102 CO2 / 145 O2 = 0.7
Q.4. Where is respiratory electron transport system (ETS) located in plants ? (2019)
(a) Mitochondrial matrix
(b) Outer mitochondrial membrane
(c) Inner mitochondrial membrane
(d) Intermembrane space
Q.5. What is the role of NAD+ in cellular respiration? (2018)
(a) It functions as an enzyme.
(b) It functions as an electron carrier.
(c) It is a nucleotide source for ATP synthesis.
(d) It is the final electron acceptor for anaerobic respiration.
NAD is the electron carrier that picks the electron from other molecules and reduced to form NADH. It is the used as reducing agent to donate electrons.
Q.6. Which of these statements is incorrect? (2018)
(a) Enzymes of TCA cycle are present in mitochondrial matrix.
(b) Glycolysis occurs in cytosol.
(c) Glycolysis operates as long as it is supplied with NAD that can pick up hydrogen atoms.
(d) Oxidative phosphorylation takes place in outer mitochondrial membrane.
Oxidative phosphorylation is the synthesis of energy rich ATP molecules with the help of energy liberated during oxidation of reduced coenzymes (NADH, FADH2) produced in respiration. The enzyme required for this synthesis is ATP synthase. It is located in F1 or head piece of F0-F1 or elementary particles present on the inner mitochondrial membrane.
Q.7. Which statement is wrong for Krebs’ cycle? (2017)
(a) There is one point in the cycle where FAD+ is reduced to FADH2.
(b) During conversion of succinyl CoA to succinic acid, a molecule of GTP is synthesised.
(c) The cycle starts with condensation of acetyl group (acetyl CoA) with pyruvic acid to yield citric acid.
(d) There are three points in the cycle where NAD+ is reduced to NADH+ H+.
Krebs’ cycle starts with condensation of acetyl group (acetyl CoA) with oxaloacetate to form a tricarboxylic, 6-carbon compound called citric acid.
Q.8. Which of the following biomolecules is common to respiration-mediated breakdown of fats, carbohydrates and proteins? (2016)
(b) Fructose 1, 6-bisphosphate
(c) Pyruvic acid
(d) Acetyl CoA
Carbohydrates are usually first converted into glucose before they are used for respiration. Fats are broken down into glycerol and fatty acids first. If fatty acids were to be respired they would first be degraded to acetyl CoA and enter the pathway. Glycerol would enter the pathway after being converted to 3-phosphoglyceraldehyde (PGAL). The proteins are degraded by proteases to individual amino acids (after deamination) and depending on their structure enter the pathway within the Krebs cycle or as pyruvate or acetyl CoA. Thus, acetyl CoA is the common metabolite of all the three (carbohydrates, proteins and fats).
The given flowchart shows these interrelationships:
Q.9. Oxidative phosphorylation is (2016)
(a) Formation of ATP by transfer of phosphate group from a substrate to ADP
(b) Oxidation of phosphate group in ATP
(c) Addition of phosphate group to ATP
(d) Formation of ATP by energy released from electrons removed during substrate oxidation.
Oxidative phosphorylation is the synthesis of energy rich ATP molecules with the help of energy liberated during oxidation of reduced co-enzymes (NADU, FADH2) produced in respiration.
The enzyme required for this synthesis is called ATP synthase. It is located in F1 or head piece of F0 - F1 or elementary particles present in the inner mitochondrial membrane. F1 particle is capable of ATP synthesis.
ATP synthase becomes active in ATP formation only when there is a proton gradient having higher concentration of H+ or protons on the F0 side as compared to F1 side. This higher concentration creates an electric potential across the mitochondrial membrane. The proton gradient and membrane electric potential together form proton motive force (PMF). The flow of protons through the F0 channel which induces F1 particle to function as ATP synthase. The energy of the proton gradient is used in attaching a phosphate radicle to ADP by high - energy bond. This produces ATP.
Q.10. Specialised epidermal cells surrounding the guard cells are called (2016)
(a) Complementary cells
(b) Subsidiary cells
(c) Bulliform cells
Specialized epidermal cells surrounding the guard cells are known as Subsidiary or accessory cell.
Q.11. In which one of the following processes CO2 in not released? (2014)
(a) Aerobic respiration in plants
(b) Aerobic respiration in animals
(c) Alcoholic fermentation
(d) Lactate fermentation
Lactic acid fermentation: It occurs in lactic acid bacteria (Lactobacillus) and muscles (Human). Pyruvic acid produced in glycolysis is reduced by NADH2 to form lactic acid without producing carbon dioxide.