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All questions of Biotechnology & its Applications for NEET Exam

Main objective of production/use of herbicide resistant GM crops is to
[2008]
  • a)
    eliminate weeds from the field without the use of manual labour
  • b)
    eliminate weeds from the field without the use of herbicides
  • c)
    encourage eco-friendly herbicides
  • d)
    reduce herbicide accumulation in food articles for health safety 
Correct answer is option 'D'. Can you explain this answer?

Ayush Sengupta answered
Main objective of production/use of herbicide resistant GM crops is to reduce herbicide accumulation in food articles for health safety. GM plants has been useful in many ways. Genetic modifications has made crops more tolerant to abiotic stresses, reduced reliance on chemical pesticides, enhanced nutritional value of food. 

 A technology which has found immense use in solving cases of disputed parentage is
  • a)
    Monoclonal antibody production
  • b)
    DNA finger printing
  • c)
    Polymerase chain reaction
  • d)
    Recombinant DNA technology
Correct answer is option 'B'. Can you explain this answer?

Mohammad Karim answered
Option B - for exapmple for identifying the true father DNA samples of child, mother and possible fathers are taken and their DNA fingerprints are obtained. the print of child DNA Match to the prints of biological parents

The American company which is involved in the manufacturing of Humulin is
  • a)
    Elixir
  • b)
    Ranbaxy
  • c)
    Prohealth
  • d)
    Eli Lily
Correct answer is option 'D'. Can you explain this answer?

Harshitha Dey answered
In 1983, the American company Eli Lily prepared two DNA sequences corresponding to A and B and introduced into plasmid of E.coli to produce insulin chain.

The term ‘Humulin’ is used for
  • a)
    Hydrolytic enzyme
  • b)
    Isoenzyme
  • c)
    Human insulin
  • d)
    Antibiotic
Correct answer is option 'C'. Can you explain this answer?

Rohan Singh answered
Humulin is human insulin used for treating diabetes. Prior to its development, diabetics used insulin isolated from pig and cow pancreases. Developed by Genentech, the first American biotechnology company, Humulin was licensed to Eli Lilly and became the first marketable product created through recombinant DNA technology. Its licensing by the FDA in October 1982 also made it the first recombinant pharmaceutical approved for use in the United States.

 Cultivation of Bt cotton has been much in the news. The prefix Bt means
  • a)
    “Barium treated” cotton seeds
  • b)
    “Bigger thread” variety of cotton with better tensile strength
  • c)
    Carrying an endotoxin gene from Bacillus thuringiensis
  • d)
    Produced by “biotechnology” using restriction enzymes and ligases
Correct answer is option 'C'. Can you explain this answer?

Rajeev Saxena answered
Bt-cotton, Bt-tobacco, Bt-tomato etc., are transgenic plants having Bt-2 gene encoding Bt toxin (e.g., Thurioside). Bt-toxin gene has been isolated from a bacteria Bacillus thuringiensis there for called Bt. (i.e., Bacillus thuringiensis). These plants are resistant for more than 140 species of insects including common cabbage worm, tent caterpillars, bag worms, canker worms, gypsy worm etc.

Which of the following is not applicable for a biopatent?
  • a)
    Formation of new products
  • b)
    Unearthing the DNA sequences
  • c)
    Discovery of cell lines
  • d)
    Using the existing technology to produce new products
Correct answer is option 'C'. Can you explain this answer?

Geetika Shah answered
A biological patent is a patent on an invention in the field of biology that by law allows the patent holder to exclude others from making, using, selling, or importing the protected invention for a limited period of time. Hence, the correct option is c.

In history of biology, human genome project led to the development of :
[2011M]
  • a)
    biotechnology
  • b)
    biomonitoring
  • c)
    bioinformatics
  • d)
    biosystematics
Correct answer is option 'C'. Can you explain this answer?

Diya Datta answered
Common activities in bioinformatics include mapping and analysing DNA and protein sequences, aligning different DNA etc are the part of human genome project.

Hybridoma technology has been successfully used in
  • a)
    Production of alcohol in bulk
  • b)
    Synthesis of monoclonal antibodies
  • c)
    Synthesis of haemoglobin
  • d)
    Production of somatic hybrids
Correct answer is option 'B'. Can you explain this answer?

Rajat Kapoor answered
Hybridoma technology has contributed to virtually all areas of biology and medicine and set the scene for important advances in cell biology and immunodiagnostics. Blood transfusion and transfusion medicine have benefited significantly from development in hybridoma technology, and this has resulted in the generation of a wide range of monoclonal antibodies to human blood group antigens. These antibodies have also had an impact on diagnostic techniques such as enzyme-linked immunosorbent assay and flow cytometry as well as on the application of therapeutic agents in the clinical environment. However, rapid advances in the development of knockout and transgenic mice as well as in nucleic acid-based immunization may well lead hybridoma technology into a new era.

Which of the following Bt crops is being grown in India by the farmers?
[NEET 2013]
  • a)
    Cotton
  • b)
    Brinjal
  • c)
    Soyabean
  • d)
    Maize
Correct answer is option 'A'. Can you explain this answer?

Harshitha Dey answered
Bt cotton is being grown in India by the farmers. Bt cotton is pest resistant plant which could decreased the pesticides used. Bt toxin is produced by a bacterium Bacillus thusringiensis (Bt for short). Bt toxin gene has been cloned from the bacteria and been expressed in  plants to provide resistance to insects without the need for insecticides.

 Insect resistance transgenic cotton has been produced by inserting a piece of DNA from
  • a)
    A virus
  • b)
    A bacterium
  • c)
    A wild relative of cotton
  • d)
    An insect
Correct answer is option 'B'. Can you explain this answer?

Syed Saif answered
Bacillus killing the larvae of flour moths in Germany and published a description of the bacterium and its … Mycogen, another small biotechnology firm, instead cloned Bt genes and expressed them in an alternate host, Pseudomonas fluorescens, a bacterial species common in transgenic cotton.

Continuous addition of sugars in ‘fed batch’ fermentation is done to:
[2011]
  • a)
    produce methane
  • b)
    obtain antibiotics
  • c)
    purify enzymes
  • d)
    degrade sewage
Correct answer is option 'C'. Can you explain this answer?

A fed batch is a biotechnological batch process which is based on feeding of a growth limiting nutrient substrate to culture. It is done for purifying enzymes.

A transgenic food crop which may help in solving the problem of night blindness in developing countries is
  • a)
    Bt soybean
  • b)
    Flavrsavr tomatoes
  • c)
    Golden rice
  • d)
    Starlink maize
Correct answer is option 'C'. Can you explain this answer?

Chirag Ghosh answered
Golden Rice as a solution to night blindness

Golden Rice is a genetically modified rice variety that has been developed to help combat vitamin A deficiency, which can lead to night blindness and other health problems in developing countries. The rice is genetically modified to produce beta-carotene, a precursor to vitamin A, which is not naturally found in traditional rice varieties.

How does Golden Rice work?

Golden Rice is genetically engineered to produce beta-carotene in the endosperm of the rice grain, which is the part of the plant that is eaten. Beta-carotene is a pigment that gives fruits and vegetables their orange or yellow color, and it is also a precursor to vitamin A. When humans consume beta-carotene, it is converted to vitamin A in the body.

Why is Golden Rice important?

Vitamin A deficiency is a serious health problem in many developing countries, particularly in Southeast Asia and Africa. According to the World Health Organization, an estimated 250 million preschool children are vitamin A deficient, and it is estimated that between 250,000 and 500,000 malnourished children become blind every year due to vitamin A deficiency. Golden Rice is one potential solution to this problem, as it provides a source of beta-carotene that can be converted to vitamin A in the body.

Conclusion

Golden Rice is a promising solution to the problem of vitamin A deficiency in developing countries, which can lead to night blindness and other health problems. While there is still some controversy surrounding genetically modified crops, Golden Rice has the potential to make a significant impact on the health and well-being of millions of people around the world.

A radioactively labeled Probe can be detected by
  • a)
    Autoradiography
  • b)
    UV rays
  • c)
    ELISA
  • d)
    PCR
Correct answer is option 'A'. Can you explain this answer?

Priyanka Iyer answered
Radioactively labeled probe can be detected by autoradiography. A single stranded DNA or RNA tagged with a radioactive molecule is allowed to hybridise to its complementary DNA in a clone of cells followed by detecting using autoradiography.

Bacillus thuringiensis (Bt) strains have been used for designing novel :
[2005]
  • a)
    Biofertilizers
  • b)
    Bio-metallurgical techniques
  • c)
    Bio-mineralization processes
  • d)
    Bioinsecticidal plants
Correct answer is option 'D'. Can you explain this answer?

Moumita Khanna answered
Bt cotton, a transgenic crop variety has been introduced in India. The Bt cotton variety contains a foreign gene obtained from Bacillus thuringiensis. This bacterial gene protects cotton from the ball worm, a major pest of cotton.

The most common substrate used in distilleries for the production of ethanol is
[2011]
  • a)
    corn meal
  • b)
    soya meal
  • c)
    ground gram
  • d)
    molasses
Correct answer is option 'D'. Can you explain this answer?

Molasses are commonly used in distillaries for the production of ethanol. The molasses is diluted to a mash containing 10-20 wt % of sugar. By the fermentation system of molasses ethanol can be produced.

Read the given statements and select the correct option.
Statement 1: Transgenic mouse is termed as 'super mouse' because it is twice big in size than the normal mouse.
Statement 2: In 'super mouse', the gene for human growth factor has been introduced and expressed.
  • a)
    Both statements 1 and 2 are correct and statement 2 is correct explanation of statement 1 
  • b)
    Statement 1 is correct but statement 2 is incorrect but statement 2 is not the correct explanation of statement 1
  • c)
    Statement 1 is incorrect but statement 2 is correct
  • d)
    Both statements 1 and 2 are incorrect
Correct answer is option 'A'. Can you explain this answer?

Priya Menon answered
Statement 1 is incorrect but statement 2 is correct
Transgenic mice are called "super mice" because they have been genetically modified to express certain traits or genes that are not found in normal mice. The term "super mouse" does not refer to the size of the mouse, but rather to the fact that it has been genetically modified. In statement 2, it is correct that In 'super mouse', the gene for human growth factor has been introduced and expressed. This is one of the reasons why transgenic mice are often used in scientific research, as they can be used to model human diseases or to study the effects of certain drugs or therapies.

The genetically-modified (GM) brinjal in India has been developed for:
[2010]
  • a)
    insect-resistance
  • b)
    enhancing shelf life
  • c)
    enhancing mineral content
  • d)
    drought-resistance
Correct answer is option 'A'. Can you explain this answer?

Mahesh Saini answered
The genetically modified brinjal in India has been developed for insect resistance. Bt brinjal is a transgenic brinjal that is developed by inserting a crystal gene from the Bacillus thuringiensis into the brinjal’s genome. This process of insertion is accomplished using Agrobacterium mediated recombination.

ELISA is used to detect viruses where the key reagent is
[2003]
  • a)
    RNase
  • b)
    alkaline phosphatase
  • c)
    catalase
  • d)
    DNA probe
Correct answer is option 'B'. Can you explain this answer?

ELISA test is a technique which can detect any amount of an antibody or antigen with the help of an enzyme. The commonly used enzymes are alkaline phosphatase and peroxidase.

Insulin earlier was extracted from
  • a)
    Fishes
  • b)
    Turkeys
  • c)
    whales
  • d)
    Slaughtered cattle and pigs
Correct answer is option 'D'. Can you explain this answer?

Harshitha Dey answered
Earlier time insulin required for controlling blood sugar was obtained from slaughtered cattle and pigs. This insulin caused allergy in diabetic patients.

First gene therapy was done for
  • a)
    Down’s syndrome
  • b)
    Alkaptonuria
  • c)
    ADA deficiency
  • d)
    Cystic fibrosis
Correct answer is option 'C'. Can you explain this answer?

Shounak Nair answered
First gene therapy was done in 1990 to a 4 year old girl with a ADA deficiency. ADA enzyme is essential for proper functioning of immune system.

Which variety of rice was patented by a U.S. company even though the highest number of varieties of this rice is found in India?
  • a)
    Sharbati Sonara
  • b)
    Co-667
  • c)
    Basmati
  • d)
    Lerma Roja
Correct answer is option 'C'. Can you explain this answer?

Priya Sen answered
Introduction
The patenting of rice varieties has sparked significant debate, particularly when a U.S. company patents a variety predominantly found in countries like India. Among these varieties, Basmati rice stands out as a notable example.
What is Basmati Rice?
- Basmati rice is known for its unique aroma, long grains, and fluffy texture.
- It has been cultivated in the Indian subcontinent for centuries and is a staple in various traditional dishes.
Patenting Issues
- In the late 1990s, a U.S. company, RiceTec, patented hybrid varieties that they claimed to be Basmati.
- This patent raised concerns among Indian farmers and stakeholders who argued that Basmati is a traditional variety that has been cultivated for generations in India and Pakistan.
Consequences of Patenting
- The patent led to legal battles over the rights to the name "Basmati."
- It highlighted the complexities of intellectual property rights in agriculture, especially concerning indigenous crops.
Conclusion
The case of Basmati rice serves as a significant example of the intersection between traditional agricultural practices and modern patent laws. Despite the patenting attempts, the highest number of Basmati rice varieties continues to be cultivated in India, emphasizing the importance of preserving indigenous agricultural heritage while navigating the challenges posed by globalization and intellectual property rights.

The first transgenic cow was
  • a)
    Rosie
  • b)
    Dolly
  • c)
    Siana
  • d)
    Verra
Correct answer is option 'A'. Can you explain this answer?

Vaibhav Basu answered
The first transgenic cow is Rosie which produced human protein riched milk having alpha-lactalbumin, which is more balanced than conventional cow’s milk.

Silencing of mRNA has been used in producing transgenic plants resistant to:
[2011M]
  • a)
    bollworms
  • b)
    nematodes
  • c)
    white rusts
  • d)
    bacterial blights
Correct answer is option 'B'. Can you explain this answer?

In this technique nematode specific genes are introduced in the host plant in such a way that it produces both sense and antisense RNA. The two RNA’s being complementary to each other from a double stranded RNA (dsRNA) which is also called interfering RNA responsible for initiating RNA interference (RNA i). This (dsRNA) bind to and prevent translation of specific mRNA of nematode (gene silencing). Thus transgenic plants based on RNAi technology are resistant to nematode.

Cry I Ab controls
  • a)
    Stem Borer
  • b)
    Corn Borer
  • c)
    Cotton Bollworm
  • d)
    Root pest
Correct answer is option 'B'. Can you explain this answer?

Harshitha Dey answered
Specific Bt toxin gene is isolated from Bacillus thuringiensis and incorporated into several crop plants. The protein encoded by gene CryI Ab controls corn borer.

Gene can therapy can be referred to as
  • a)
    pre - clinical testing for inherited diseases in newborns
  • b)
    treatment of diseases caused by genetic defect
  • c)
    genetic engineering using rDNA technology
  • d)
    cancer treatment using in vitro cultured stem cells
Correct answer is option 'B'. Can you explain this answer?

Geetika Shah answered
Gene therapy is a collection of methods, that allows correction of gene defect that has been diagnosed in a child/embryo. Correcting of a genetic defect involves delivery of a normal gene into the individual or embryo to take over the function of and compensate for the non-functional gene.

Rules of conduct that may be used to regulate our activities in relation to the biological world is called
  • a)
    bioethics
  • b)
    biowar
  • c)
    biopatent
  • d)
    biopiracy
Correct answer is option 'A'. Can you explain this answer?

Suresh Iyer answered
Bioethics may be viewed as the set of standards that may be used to regulate various activities based on their effects on the biological world. This is because biotechnology has aroused social as well as political concerns, which have ranged from biotechnology being unnatural to detrimental to biodiversity.

Biotechnology can be defined as the use of biological processes in industry and technology. During the past three decades biotechnology has gained attention due to the gene cloning process. Biotechnological applications are playing an important role in agriculture. They are used in agro-chemical based agriculture, in organic farming, genetically
engineered crop-based agriculture. The green revolution is the milestone or result of these biotechnological processes.
Q. GEAC stands for _________ .
  • a)
    Genetic Extension Approval Committee
  • b)
    Genetic Engineering Approval Committee
  • c)
    Genetic Engineering Approval Committee
  • d)
    None of the above
Correct answer is option 'B'. Can you explain this answer?

Abhay Sengupta answered
GEAC stands for Genetic Engineering Approval Committee.

The Genetic Engineering Approval Committee (GEAC) is a regulatory body in India that is responsible for granting approval for activities involving the release of genetically engineered organisms (GEOs) into the environment. It was established under the Ministry of Environment, Forest and Climate Change (MoEFCC) in accordance with the Rules for the Manufacture, Use, Import, Export and Storage of Hazardous Microorganisms/Genetically Engineered Organisms or Cells (1989) under the Environment Protection Act, 1986.

Role of GEAC:
The primary role of GEAC is to ensure the safe use and handling of genetically engineered organisms in order to protect human health and the environment. Some of the key responsibilities of GEAC include:

1. Evaluation and approval of proposals: GEAC evaluates applications for the release of GEOs and grants approvals based on scientific assessments of their potential risks and benefits. The committee considers factors such as the nature of the organism, the intended use, and the potential environmental impact.

2. Monitoring and surveillance: GEAC monitors the implementation of approved projects to ensure compliance with the conditions and guidelines set forth during the approval process. This includes regular inspections, data collection, and reporting.

3. Risk assessment: GEAC conducts risk assessments to evaluate the potential adverse effects of genetically engineered organisms on human health and the environment. This involves analyzing data on the characteristics of the organism, its potential for gene transfer, and its potential to cause harm.

4. Public consultation: GEAC organizes public hearings and consultations to gather feedback and opinions from stakeholders, including scientists, environmentalists, farmers, and the general public. This helps in ensuring transparency and public participation in decision-making processes.

Conclusion:
In summary, the Genetic Engineering Approval Committee (GEAC) plays a crucial role in regulating and monitoring the release of genetically engineered organisms in India. By evaluating proposals, conducting risk assessments, and ensuring compliance with guidelines, GEAC helps to ensure the safe and responsible use of biotechnology in agriculture and other sectors.

Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :
Assertion (A) : Norman E. Borlaug was the father of "Green revolution".
Reason (R) : Green revolution is the period when there is significant increase in agricultural productivity of grains (specially wheat and rice).
  • a)
    Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).
  • b)
    Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).
  • c)
    Assertion (A) is true but reason (R) is false.
  • d)
    Assertion (A) is false but reason (R) is true.
Correct answer is option 'A'. Can you explain this answer?

Riya Banerjee answered
The Green Revolution was an endeavour initiated by Norman Borlaug in the 1960s. He is known as the 'Father of Green Revolution' in world. It led to him winning the Nobel Peace Prize in 1970 for his work in developing High Yielding Varieties (HYVs) of wheat. In India, the Green Revolution was mainly led by M.S. Swaminathan. The Green Revolution resulted in a great increase in production of food grains (especially wheat and rice) due to the introduction into developing countries of new, high-yielding variety seeds, beginning in the mid-20th century. Its early dramatic successes were in Mexico and the Indian subcontinent. The Green Revolution, spreading over the period from 1967-68 to 1977-78, changed India’s status from a food-deficient country to one of the world's leading agricultural nations.

Sheep Dolly was genetically similar to
  • a)
    The mother from which nucleated fertilized egg was taken
  • b)
    The mother from which the nucleus of udder cell was taken
  • c)
    The surrogate mother
  • d)
    Both surrogate mother and nuclear donor mother
Correct answer is option 'B'. Can you explain this answer?

Mira Joshi answered
Dolly, the sheep, was cloned in 1997, Ian wilmur and his colleagues at the Roslin Research Scotland, took cells from ewe (mother sheep’s udder) They stored these udder cells in low nutrient culture. This stopped the starved cells from dividing switched of their active genes. One compete udder cell with its nucleus was selected, because its nucleus carries the mother's genetic information in the mean time, an unfertilised egg cell was taken from a different ewe. Its nucleus was removed leaving an empty cell containing all the essential machinery to produce an embryo. They then fused udder cell nucleus with the empty egg cell by electrical stimulation. The altered egg was cultured for six days.
Out of many resulting embryos, one was implanted in the uterus of the surrogate mother, where it grew into a lamb, So Dolly was born, genetically identical to mother sheep because her first cell nucleus came from mother’s cell. Dolly's birth proved that adult cells could acquire the cloning potential of embryonic cells.

Early detection of a disease is possible by
  • a)
    PCR
  • b)
    Gene therapy
  • c)
    Recombinant DNA technology and ELISA
  • d)
    both (a) and (c)
Correct answer is option 'D'. Can you explain this answer?

Raghav Bansal answered
Using conventional methods of diagnosis (serum and urine analysis, etc.) early detection is not possible. Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis.

A monopoly granted to a person who has either invented a new and useful article, made improvement in an existing article or invented a new process of making an article is called
  • a)
    biopiracy
  • b)
    bioethics
  • c)
    patent
  • d)
    genetic modification
Correct answer is option 'C'. Can you explain this answer?

Ishan Roy answered
Understanding Patents
A patent is a legal right granted to an inventor, providing exclusive rights to make, use, sell, or distribute an invention for a specified period, typically 20 years. This protection encourages innovation by allowing inventors to benefit from their creations.
Key Features of Patents
- Inventiveness: To qualify for a patent, the invention must be new, useful, and non-obvious. This means that it should not be something that anyone skilled in the field could easily deduce.
- Types of Innovations: Patents can be granted for various types of inventions, including:
- New and useful articles
- Improvements to existing products
- New processes for manufacturing or creating products
- Legal Protection: A patent holder can prevent others from making, using, or selling the invention without permission. This exclusivity allows inventors to recoup their investment in research and development.
Importance of Patents
- Encouraging Innovation: The patent system incentivizes inventors to develop new products and processes, fostering a culture of innovation.
- Economic Growth: By protecting intellectual property, patents contribute to economic development by allowing businesses to create jobs and introduce new technologies.
- Public Disclosure: In exchange for exclusive rights, inventors must publicly disclose their inventions, which facilitates knowledge sharing and further innovation.
In summary, a patent is crucial for safeguarding the rights of inventors while promoting technological advancement, which is why option 'C' is the correct answer.

Agrochemical based agriculture includes
  • a)
    Fertilisers and pesticides
  • b)
    Genetically modified crops
  • c)
    RNA interference
  • d)
    All of these
Correct answer is option 'A'. Can you explain this answer?

Gaurav Kumar answered
Agrochemical based agriculture is used to increase the food production. It includes use of agrochemicals such as fertilisers and pesticides.

Which of the following companies started selling humulin in the year 1983?
  • a)
    Eli Lilly
  • b)
    Genetech
  • c)
    GEAC
  • d)
    None of these
Correct answer is option 'A'. Can you explain this answer?

Answer:

Eli Lilly is the company that started selling Humulin in the year 1983.

Explanation:

Humulin is a type of insulin used to treat diabetes, and it was the first genetically engineered drug to be approved by the US Food and Drug Administration (FDA). The development of Humulin was a joint effort between Eli Lilly and Genentech, but Eli Lilly was the company responsible for marketing and selling the drug.

The approval of Humulin in 1982 marked a significant milestone in the field of biotechnology and paved the way for the development of more genetically engineered drugs. Eli Lilly has since continued to be a major player in the diabetes market, developing and marketing other insulin products such as Humalog and Basaglar.

In summary, Eli Lilly started selling Humulin in the year 1983, making it the first genetically engineered drug to be marketed and sold to the public.

Which of the following has been covered under the broad patent category?
  • a)
    Triticum
  • b)
    Oryza
  • c)
    Pissum sativum
  • d)
    Brassica
Correct answer is option 'B'. Can you explain this answer?

Preethi Saha answered
Broad patent category refers to patents that cover a large category of organisms or genetic material. In this case, the correct answer is option 'B' - Oryza.

Explanation:

- Triticum: It is a genus of plants that includes wheat species. However, it is not a broad patent category as it refers to a specific genus of plants.
- Pissum sativum: It is the scientific name for peas. Similar to Triticum, it refers to a specific plant species and not a broad patent category.
- Brassica: It is a genus of plants that includes several species such as mustard, cabbage, and broccoli. However, it is not a broad patent category as it refers to a specific genus of plants.
- Oryza: It is a genus of plants that includes several species of rice. Oryza has been covered under the broad patent category as it refers to a large category of organisms.

In conclusion, the correct answer is option 'B' - Oryza as it has been covered under the broad patent category.

Which of the following statements regarding gene therapy is/are correct?
  • a)
    It is an application of biotechnology, in which a defective gene is manipulated by introduction of a normal, healthy and functional gene
  • b)
    The genetic disorders that are being investigated for gene therapy, range from sickle-cell anaemia to severe combined immuno-deficiency (SCID)
  • c)
    The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency
  • d)
    All of these
Correct answer is option 'D'. Can you explain this answer?

Mira Joshi answered
Gene therapy is a technique for correcting defective genes responsible for disease development. Under intensive investigation are, disease ranging from the rare genetic diseases caused by single mutations like sickle cell anaemia to killer diseases such as server combined immuno-deficiency (SCID). The first clinical gene therapy was given in 1990 to a 4-years old girl with adenosine deaminase (ADA) deficiency. This enzymes is very important for the immune system to function. ADA deficiency can lead to severe combined immuno-deficiency (SCID).

Study the following statements regarding Bt toxins produced by bacterium Bacillus thuringiensis and select the correct one.
  • a)
    Most strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans, coleopterans and dipterans
  • b)
    Bt toxin proteins do not kill the bacteria themselves because the toxin proteins occur in an inactive form called protoxins
  • c)
    When an insect ingests the inactive Bt toxin, it is converted to an active form of toxin due to alkaline pH of the gut which solubilises the protein toxin crystals
  • d)
    All of these
Correct answer is option 'D'. Can you explain this answer?

Ajay Yadav answered
Soil bacterium, Bacillus thuringiensis (Bt) produces proteins that kill certain insects like lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). Bacillus thuringiensis forms some intracellular protein crystals. These crystals contain a toxic insecticidal protein. The Bt toxin protein exists as inactive toxin and is converted into an active form due to the alkaline pH of the alimentary canal that solubilises the crystals. The activated toxin binds to the surface of midgut epithelial cells and create pores which cause cell swelling and lysis and finally cause death of the insect.

Which one of the following is commonly used in transfer of foreign DNA into crop plants?
[2009]
  • a)
    Meloidogyne incognita
  • b)
    Agrobacterium tumefaciens
  • c)
    Penicillium expansum
  • d)
    Trichoderma harzianum
Correct answer is option 'B'. Can you explain this answer?

Shounak Nair answered
Agrobacterium tumefaciens is the causal agent of crown gall disease (the formation of tumours) in over 140 species of dicot. This disease caused by a DNA plasmid (Ti plasmid) carried by bacterium and transferred to the plant cells. Ti plasmid has widely used in plant engineering as a vector in order to inject gene in host plant to form transgenic plant.

Read the given statements and select the correct options
Statement 1: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Statement 2: Both bacteria and yeast multiply very fast to form huge population which expresses the desired gene.
  • a)
    Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.
  • b)
    Both Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.
  • c)
    Statement 1 is correct and statement 2 is incorrect.
  • d)
    Both statements 1 and 2 are incorrect.
Correct answer is option 'A'. Can you explain this answer?

EduRev NEET answered
Recombinant DNA is a form of DNA whose nucleotide sequence has undergone alteration as a result of incorporation of exchange with another DNA strand. In recombinant DNA technology, human genes are often transferred into bacteria or yeast because both bacteria and yeast multiply very fast to form huge population, which expresses the desired gene. By doing so, one can observe the phenotypes of the gene more quickly and in a larger scale than by studying the same traist in humans.

For which of the disease transgenic models do not exists now days?
  • a)
    Tuberculosis
  • b)
    Cancer
  • c)
    Cystic fibrosis
  • d)
    Alzheimer’s disease.
Correct answer is option 'A'. Can you explain this answer?

Priyanka Iyer answered
Transgenic model for treatment of disease is available today for cancer, cystic fibrosis, Alzheimer’s disease and rheumatoid arthritis disease.

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