A radioactively labeled Probe can be detected bya)Autoradiographyb)UV ...
Radioactively labeled probe can be detected by autoradiography. A single stranded DNA or RNA tagged with a radioactive molecule is allowed to hybridise to its complementary DNA in a clone of cells followed by detecting using autoradiography.
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A radioactively labeled Probe can be detected bya)Autoradiographyb)UV ...
Radiolabelled probe can be detected by autoradiography and the location of the target sequence in the bands in the original gel determined. Southern blots are used for DNA analysis. Northern blots are used for RNA analysis.
A radioactively labeled Probe can be detected bya)Autoradiographyb)UV ...
Radioactively Labeled Probe Detection:
Autoradiography:
Autoradiography is a technique used to visualize the location of radioactively labeled molecules in a sample. In the context of a radioactively labeled probe, autoradiography can be used to detect the presence of the probe in a biological sample.
- Autoradiography involves exposing the sample to a photographic film or an imaging plate.
- The radioactively labeled probe emits radiation in the form of radioactive decay.
- This radiation exposes the photographic film or imaging plate, resulting in dark spots or bands.
- The dark spots or bands correspond to the location of the radioactively labeled probe in the sample.
- By analyzing the autoradiograph, researchers can determine the presence and location of the radioactively labeled probe.
UV Rays:
UV rays, or ultraviolet rays, are not typically used for the detection of radioactively labeled probes. UV rays are a form of electromagnetic radiation with a shorter wavelength than visible light.
- UV rays are commonly used for various applications such as DNA visualization in gel electrophoresis.
- However, UV rays do not have the ability to detect radioactively labeled probes directly.
- Radioactive labeling involves the incorporation of a radioactive isotope, which emits radiation that can be detected by other methods such as autoradiography.
ELISA:
ELISA, or enzyme-linked immunosorbent assay, is a technique used to detect and quantify the presence of specific molecules in a sample. However, ELISA is not specifically designed for the detection of radioactively labeled probes.
- ELISA typically relies on the use of enzymes and antibodies to detect specific molecules.
- It can be used to detect proteins, antibodies, and other molecules of interest.
- Radioactively labeled probes do not involve the use of enzymes or antibodies, making ELISA less suitable for their detection.
PCR:
PCR, or polymerase chain reaction, is a technique used to amplify specific DNA sequences. It is not directly used for the detection of radioactively labeled probes.
- PCR involves multiple cycles of DNA denaturation, primer annealing, and DNA synthesis.
- It is commonly used to amplify and detect specific DNA sequences.
- However, PCR does not directly detect radioactively labeled probes.
- After PCR amplification, the presence of radioactively labeled probes can be detected using autoradiography or other appropriate methods.
Conclusion:
The correct answer is option 'A' - Autoradiography. Autoradiography is specifically designed for the detection of radioactively labeled probes by visualizing the location of the probe in a sample through exposure to a photographic film or imaging plate. UV rays, ELISA, and PCR are not directly used for the detection of radioactively labeled probes.