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The introduction of t-DNA into plants involves: [2015 RS]
- a)Altering the pH of the soil, then heat shocking the plants
- b)Exposing the plants to cold for a brief period
- c)Allowing the plant roots to stand in water
- d)Infection of the plant by Agrobacterium tumefaciens
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
The introduction of t-DNA into plants involves: [2015 RS]a)Alter...
(d) Agrobacterium tumefaciens introduces tDNA into the plant.
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The introduction of t-DNA into plants involves: [2015 RS]a)Alter...
Introduction of t-DNA in plants through Agrobacterium tumefaciens infection
Agrobacterium tumefaciens is a soil bacterium that is capable of transferring a portion of its DNA, known as transfer DNA (t-DNA), into the genome of plants. This process is known as Agrobacterium-mediated transformation and is widely used in plant biotechnology for the introduction of new traits or genes into plants. The steps involved in this process are:
1. Infection of the plant by Agrobacterium tumefaciens
The first step in Agrobacterium-mediated transformation is the infection of the plant by the bacterium. Agrobacterium tumefaciens has the ability to infect a wide range of plant species, including dicots and some monocots. The bacterium enters the plant through wounds or natural openings such as stomata.
2. Transfer of t-DNA into the plant cell
Once inside the plant, Agrobacterium tumefaciens transfers a portion of its DNA, known as t-DNA, into the genome of the plant cell. The t-DNA is flanked by sequences known as border sequences, which are recognized by the bacterium's virulence proteins. The t-DNA is then transported across the plant cell membrane and integrated into the plant genome.
3. Expression of the introduced gene
The t-DNA can carry one or more genes of interest, which can be expressed in the plant cell. The expression of the introduced gene can result in the production of a new protein or the modification of an existing protein, leading to the development of a new trait in the plant.
4. Regeneration of the transformed plant
In order to produce a whole plant with the introduced gene, the transformed plant cell must be regenerated into a whole plant. This can be achieved through the use of tissue culture techniques, which involve growing the transformed cells on a nutrient medium containing plant hormones.
Conclusion
Agrobacterium-mediated transformation is a powerful tool in plant biotechnology, allowing the introduction of new traits or genes into plants. This process involves the infection of the plant by Agrobacterium tumefaciens, the transfer of t-DNA into the plant cell, the expression of the introduced gene, and the regeneration of the transformed plant.
Agrobacterium tumefaciens is a soil bacterium that is capable of transferring a portion of its DNA, known as transfer DNA (t-DNA), into the genome of plants. This process is known as Agrobacterium-mediated transformation and is widely used in plant biotechnology for the introduction of new traits or genes into plants. The steps involved in this process are:
1. Infection of the plant by Agrobacterium tumefaciens
The first step in Agrobacterium-mediated transformation is the infection of the plant by the bacterium. Agrobacterium tumefaciens has the ability to infect a wide range of plant species, including dicots and some monocots. The bacterium enters the plant through wounds or natural openings such as stomata.
2. Transfer of t-DNA into the plant cell
Once inside the plant, Agrobacterium tumefaciens transfers a portion of its DNA, known as t-DNA, into the genome of the plant cell. The t-DNA is flanked by sequences known as border sequences, which are recognized by the bacterium's virulence proteins. The t-DNA is then transported across the plant cell membrane and integrated into the plant genome.
3. Expression of the introduced gene
The t-DNA can carry one or more genes of interest, which can be expressed in the plant cell. The expression of the introduced gene can result in the production of a new protein or the modification of an existing protein, leading to the development of a new trait in the plant.
4. Regeneration of the transformed plant
In order to produce a whole plant with the introduced gene, the transformed plant cell must be regenerated into a whole plant. This can be achieved through the use of tissue culture techniques, which involve growing the transformed cells on a nutrient medium containing plant hormones.
Conclusion
Agrobacterium-mediated transformation is a powerful tool in plant biotechnology, allowing the introduction of new traits or genes into plants. This process involves the infection of the plant by Agrobacterium tumefaciens, the transfer of t-DNA into the plant cell, the expression of the introduced gene, and the regeneration of the transformed plant.
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Community Answer
The introduction of t-DNA into plants involves: [2015 RS]a)Alter...
Agrobacterium tumefaciens a plant pathogenic bacterium that carries Ti plasmid.
It can transfer a particular segment of the tumor-inducing (Ti) plasmid into the nucleus of infected cells.
The transferred T-DNA is then integrated into the host genome and transcribed with it.
This ability of Agrobacterium tumefaciens to transfer the T-DNA in the host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into the genome of selected organisms.
Thus, the correct answer is option D.
It can transfer a particular segment of the tumor-inducing (Ti) plasmid into the nucleus of infected cells.
The transferred T-DNA is then integrated into the host genome and transcribed with it.
This ability of Agrobacterium tumefaciens to transfer the T-DNA in the host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into the genome of selected organisms.
Thus, the correct answer is option D.
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The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer?.
The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer?.
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The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer?, a detailed solution for The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer? has been provided alongside types of The introduction of t-DNA into plants involves: [2015 RS]a)Altering the pH of the soil, then heat shocking the plantsb)Exposing the plants to cold for a brief periodc)Allowing the plant roots to stand in waterd)Infection of the plant by Agrobacterium tumefaciensCorrect answer is option 'D'. Can you explain this answer? theory, EduRev gives you an
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