Polyethylene glycol method is used for[2009]a)biodiesel productionb)se...
Direct gene transfer is the transfer of naked. DNA into plant cells but the presence of rigid plant cell wall acts as a barrier to uptake. Therefore protoplasts are the favoured target for direct gene transfer. Polyethylene glycol mediated DNA uptake is a direct gene transfer method that utilizes the interaction between polyethylene glycol, naked DNA, salts and the protoplast membrane to effect transport of the DNA into the cytoplasm.
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Polyethylene glycol method is used for[2009]a)biodiesel productionb)se...
Polyethylene glycol (PEG) method is used for gene transfer without a vector.
Explanation:
• The PEG method is a technique used for the introduction of foreign DNA into cells without the use of a vector.
• This method involves the use of polyethylene glycol, which is a water-soluble polymer that can fuse the plasma membranes of two different cells.
• In this method, the foreign DNA is mixed with the recipient cells along with PEG, which creates small holes in the plasma membrane of the recipient cells and allows the foreign DNA to enter.
• This technique is commonly used in genetic engineering, where genes from one organism are transferred to another organism for different purposes like producing transgenic plants and animals for various applications.
• The PEG method is a simple and efficient method of gene transfer without the use of a vector, making it a popular technique in genetic engineering.
Therefore, option D is the correct answer.
Polyethylene glycol method is used for[2009]a)biodiesel productionb)se...
Introduction of DNA into plant cells without the involvement of a biological agent and leading to stable transformation is known as direct gene transfer. There are various methods for direct gene transfer, one of which is chemical method. Certain chemicals, eg, PEG (Polyethylene Glycol), polyvinyl alcohol and calcium phosphate enhances the uptake of DNA onto the outer surface of plasmalemma and the precipitate is taken up by the endocytosis.