Pollen grain of large number of species can be stored in: a)Liquid oxygenb)Liquid carbon dioxidec)Liquid sulphur dioxided)Liquid nitrogenCorrect answer is option 'D'. Can you explain this answer?

Question

Answer

Pollen grain consists of hard covering of exine but their viability may lost with time. For Hybridisation pollen grains are collected and stored in liquid nitrogen below -196 degree Celsius temperature.

Answer

Pollen grains are stored in liquid nitrogen at -196'c because at that temperature the metabolic activity of pollen will be stoped

Answer

When something is preserved in liquid Nitrogen at various temperature then this technique is known as cryopreservation.
Eg sperm are also preserved in liquid nitrogen at -170 degree celsius
and pollen grains are preserved in liquid nitrogen at -196 degree celsius.

Answer

D

Answer

The correct answer is option 'D' - Liquid nitrogen.

Explanation:

Liquid nitrogen is widely used for the storage of pollen grains of various plant species. This is because of its extremely low temperature (-196°C or -321°F) and its ability to maintain the viability and integrity of pollen grains for long periods of time. Here is why liquid nitrogen is the preferred choice for pollen grain storage:

1. Low Temperature:

Liquid nitrogen has an extremely low boiling point, which allows it to maintain a stable low temperature. This low temperature helps in preserving the pollen grains by slowing down their metabolic activities and preventing degradation.

2. Cryopreservation:

Cryopreservation is the process of preserving cells or tissues at extremely low temperatures. Liquid nitrogen is commonly used for cryopreservation because it prevents the formation of ice crystals, which can damage the cellular structure of pollen grains. By avoiding ice crystal formation, liquid nitrogen helps in maintaining the viability and genetic integrity of the stored pollen grains.

3. Long-Term Storage:

Pollen grains can be stored in liquid nitrogen for extended periods of time without significant loss of viability. This makes it ideal for long-term storage of pollen grains for scientific research, plant breeding, and conservation purposes. The low temperature of liquid nitrogen slows down the metabolic processes of the pollen grains, allowing them to remain viable for years or even decades.

4. Easy Accessibility:

Liquid nitrogen is readily available and relatively inexpensive compared to other cryopreservation methods. It is commonly used in laboratories and research facilities, making it easily accessible for storing pollen grains of various plant species.

In conclusion, the use of liquid nitrogen for storing pollen grains is preferred due to its low temperature, ability to prevent ice crystal formation, long-term viability maintenance, and easy accessibility.

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