In transgenics, expression of transgene in target tissue is determined by

[2004]

a)
enhancer

b)
promoter

c)
reporter

d)
transgene

Correct answer is option 'B'. Can you explain this answer?

Question

In transgenics, expression of transgene in target tissue is determined by[2004]a)enhancerb)promoterc)reporterd)transgeneCorrect answer is option 'B'. Can you explain this answer?

Answer

In a transgenic organism, reporter genes are used to determine whether a particular DNA construct has been successfully introduced into a cell, organ or tissue. Whereas promoter is the region of DNA that initiates the DNA transcription, an enhancer is the sequence of DNA that enhances the transcription when binds to the specific proteic and transgene is the gene that taken from the genome of one organism and introduces into the genome of another organism.

Answer

In genetic engineering or biotechnology, the result of cloning or gene insertion  into a foreign genetic material is determined by checking the extent of expression of the reporter gene.

Answer

Expression of transgene in target tissue in transgenics
Transgenes are genes that have been artificially introduced into an organism, often for the purpose of studying gene function or creating genetically modified organisms. In transgenics, the expression of a transgene in the target tissue is crucial for achieving the desired outcome.

Promoter
The promoter is a region of DNA that initiates the transcription of a particular gene. In transgenics, the promoter plays a key role in determining where and when the transgene will be expressed. By choosing a specific promoter, researchers can control the expression of the transgene in the target tissue. Promoters can be tissue-specific, inducible, or constitutive, allowing for precise control over transgene expression.

Enhancer
Enhancers are DNA sequences that can increase the transcription of a gene when bound by specific proteins. Enhancers can work in conjunction with promoters to regulate gene expression in a tissue-specific manner. By incorporating enhancer elements into the transgene construct, researchers can enhance the expression of the transgene in the desired target tissue.

Reporter
Reporters are genes that produce a detectable signal, such as fluorescence or luminescence, when expressed. Reporters are often used in transgenics to monitor the expression of the transgene in real-time. By including a reporter gene in the transgene construct, researchers can visually confirm that the transgene is being expressed in the target tissue.
In conclusion, the expression of a transgene in the target tissue in transgenics is determined by the choice of promoter, enhancer elements, and the use of reporter genes. By carefully designing the transgene construct, researchers can control the expression of the transgene to achieve their experimental goals.

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