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If a long linear dsDNA having blunt ends with 5'GAATTC3' 3'CTTAGG5' sequences at four places is cleaved by using EcoR1, how many DNA fragments are formed with cohesive ends on both sides? 1)5 2)4 3)3 4)2?
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If a long linear dsDNA having blunt ends with 5'GAATTC3' 3'CTTAGG5' se...
3 is correct answer, As corner fragments does not have both cohesive ends
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If a long linear dsDNA having blunt ends with 5'GAATTC3' 3'CTTAGG5' se...
Answer:


Number of DNA fragments with cohesive ends on both sides: 4




Explanation:


When a long linear dsDNA having blunt ends with 5'GAATTC3' 3'CTTAGG5' sequences at four places is cleaved by EcoR1, the following happens:

  • The EcoR1 enzyme recognizes and cleaves the DNA at the sequence 5'GAATTC3'.

  • This sequence is present at four places in the original DNA.

  • Each time the DNA is cleaved, two fragments are formed - one with a 5' overhang and the other with a 3' overhang.

  • Since the original DNA has four of these sequences, it will be cleaved four times, resulting in 8 fragments.

  • However, since the question asks for the number of DNA fragments with cohesive ends on both sides, we need to consider only those fragments that have an overhang on both ends.

  • Out of the 8 fragments, only 4 have cohesive ends on both sides - the ones that were cleaved at the sequences 5'GAATTC3'.

  • The other 4 fragments have blunt ends on one end and an overhang on the other end.


Therefore, the number of DNA fragments with cohesive ends on both sides is 4.
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If a long linear dsDNA having blunt ends with 5'GAATTC3' 3'CTTAGG5' sequences at four places is cleaved by using EcoR1, how many DNA fragments are formed with cohesive ends on both sides? 1)5 2)4 3)3 4)2? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If a long linear dsDNA having blunt ends with 5'GAATTC3' 3'CTTAGG5' sequences at four places is cleaved by using EcoR1, how many DNA fragments are formed with cohesive ends on both sides? 1)5 2)4 3)3 4)2? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If a long linear dsDNA having blunt ends with 5'GAATTC3' 3'CTTAGG5' sequences at four places is cleaved by using EcoR1, how many DNA fragments are formed with cohesive ends on both sides? 1)5 2)4 3)3 4)2?.
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