A student culture a callus from the tobacco pith in sterilized minimal...
The callus consists of undifferentiated tumor like cells. If the concentration of auxin with respect to that of cytokinin ratio is changed, the cells of the callus undergo transformation to produce organs like roots or shoots. If the level of cytokinin is high as compared to the auxin level, the callus cells undergo transformation and produces shoots.
High concentrations of cytokinin generally inhibit or delay root formation and also prevent root growth. High levels of cytokinin usually causes many small shoots to be produced.
Hence, shoot would develop first from the callus.
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A student culture a callus from the tobacco pith in sterilized minimal...
The callus consists of undifferentiated tumor like cells. If the concentration of auxin with respect to that of cytokinin ratio is changed, the cells of the callus undergo transformation to produce organs like roots or shoots. If the level of cytokinin is high as compared to the auxin level, the callus cells undergo transformation and produces shoots.
High concentrations of cytokinin generally inhibit or delay root formation and also prevent root growth. High levels of cytokinin usually causes many small shoots to be produced.
Hence, shoot would develop first from the callus.
A student culture a callus from the tobacco pith in sterilized minimal...
The correct answer is option 'C', which states that shoots would develop first from the callus. Let's understand why shoots develop before other structures in this scenario:
1. Introduction:
- The student cultured a callus from the tobacco pith in a sterilized minimal nutritive medium.
- The callus is a mass of undifferentiated cells that can be induced to develop into various plant structures.
2. Role of Cytokinin and Auxin:
- Cytokinin and auxin are plant growth regulators that play crucial roles in plant development.
- Cytokinin promotes shoot growth and differentiation.
- Auxin promotes root growth and differentiation.
3. Higher Cytokinin and Lower Auxin Concentration:
- The student added more cytokinin and less auxin to the medium.
- This imbalance in the concentration of the two hormones favors shoot development over root development.
4. Explanation of Shoot Development:
- A higher concentration of cytokinin stimulates the callus cells to differentiate into shoot meristems.
- Shoot meristems are undifferentiated tissues capable of giving rise to leaves, stems, and other shoot structures.
- The presence of cytokinin in excess initiates shoot meristem formation and subsequent shoot development.
5. Delayed Root Development:
- In this scenario, the lower concentration of auxin delays root development.
- Auxin is necessary for the initiation of root meristems and subsequent root development.
- With a lower auxin concentration, the callus cells are less likely to differentiate into root meristems.
6. Other Structures:
- The question specifically asks about the first structure to develop from the callus.
- While buds may eventually develop from the shoot meristems, shoots form before buds.
- Leaves require shoot development to precede their formation as well.
In conclusion, when a callus from tobacco pith is cultured in a sterilized minimal nutritive medium with a higher concentration of cytokinin and a lower concentration of auxin, shoots would develop first. This is because cytokinin promotes shoot growth and differentiation, while auxin is necessary for root development.