Mark the INCORRECT statement about agar, a gelling agent in plant tissue culture medium?a)Not digested by plant enzymesb)It does not use in micropropagation workc)It does not react with media constituentsd)Remain stable at incubation temperatureCorrect answer is option 'B'. Can you explain this answer?

Question

Answer

Solid agar plates can support microbial growth when supplemented with appropriate nutrients or be used for antibiotic selection. Agar media is essential for the study of microorganisms and molecular biology and is widely used in the culture and detection of pathogens from contaminated food and water.

Answer

in plant tissue culture medium we mainly uses others rather than agar ..

Answer

Incorrect statement: b) It does not use in micropropagation work

Explanation:

Agar is a gelling agent commonly used in plant tissue culture medium. It is derived from seaweed and has several properties that make it suitable for use in plant tissue culture. The incorrect statement in this case is that agar does not use in micropropagation work. In reality, agar is widely used in micropropagation techniques to culture and propagate plant cells, tissues, and organs.

Importance of agar in plant tissue culture:

Agar is a polysaccharide that forms a gel when mixed with water and heated. It is an inert substance that does not react with the media constituents, making it a stable and reliable gelling agent. Agar is commonly used in plant tissue culture for the following reasons:

1. Gel formation: Agar forms a solid gel at low concentrations (typically 0.7-1.0%) when mixed with plant nutrients, vitamins, and other components. This gel provides a physical support structure for the growth and development of plant cells.

2. Uniform distribution of nutrients: Agar allows the uniform distribution of nutrients and growth regulators throughout the culture medium, ensuring consistent growth of plant tissues.

3. Resistance to degradation: Agar is not digested by plant enzymes, which allows the culture medium to remain stable over extended periods of time. This is important for long-term cultures and for the production of multiple plants through micropropagation.

4. Easy manipulation: Agar can be easily liquefied by heating and solidified by cooling, allowing the preparation of culture media and the handling of plant tissues.

5. Non-toxic: Agar is non-toxic to plant cells and tissues, making it suitable for their growth and development.

Applications of agar in micropropagation:

Micropropagation is a technique used to rapidly multiply plants by culturing small pieces of plant tissue in a nutrient-rich medium. Agar is an essential component of the culture medium in micropropagation for the following reasons:

1. Support for tissue growth: Agar provides a solid support structure for the growth and development of plant tissues, allowing them to form shoots, roots, and eventually whole plants.

2. Isolation and maintenance of tissue cultures: Agar-based media are used to isolate and maintain tissue cultures in a controlled environment. The gelled medium provides a sterile and stable platform for the growth of plant tissues.

3. Propagation of plantlets: Agar-based media are used to propagate plantlets from small explants, such as shoot tips or nodal segments. The explants are placed on the surface of the medium, and the agar provides the necessary physical support for their growth.

In conclusion, agar is an essential component of plant tissue culture medium, including micropropagation work. It provides a solid support structure, allows uniform distribution of nutrients, remains stable at incubation temperature, and is not digested by plant enzymes. Agar is widely used in micropropagation techniques to culture and propagate plant cells, tissues, and organs.

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