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Read the following text and answer the following question on the basis of the same:
The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.
Q. The cutting of DNA at specific locations became possible with the discovery of :
  • a)
    Ligases
  • b)
    Restriction enzymes
  • c)
    Probes
  • d)
    Selectable markers.
Correct answer is option 'B'. Can you explain this answer?
Verified Answer
Read the following text and answer the following question on the basi...
Restriction enzymes are the molecular scissors that cut the DNA from a specific recognition site.
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The discovery of restriction enzymes enabled the cutting of DNA at specific locations.

Restriction enzymes:
Restriction enzymes, also known as restriction endonucleases, are proteins that are produced by bacteria as a defense mechanism against foreign DNA, such as viral DNA. These enzymes have the ability to recognize specific DNA sequences and cut the DNA at those locations.

How restriction enzymes work:
- Restriction enzymes recognize specific DNA sequences, which are typically palindromic, meaning the sequence reads the same in both directions. For example, the sequence 5'-GAATTC-3' is palindromic, as it reads the same in the opposite direction (3'-CTTAAG-5').
- When a restriction enzyme encounters its specific recognition sequence, it binds to the DNA and cuts the DNA backbone at specific points within or near the recognition sequence.
- The cut made by the restriction enzyme creates sticky ends or blunt ends, depending on the type of enzyme. Sticky ends have single-stranded overhangs that can easily be joined with complementary DNA ends, while blunt ends are flush cuts without any overhangs.
- By cutting DNA at specific locations, restriction enzymes allow scientists to manipulate and study DNA fragments in the laboratory.

Importance of restriction enzymes in biotechnology:
- Restriction enzymes are essential tools in genetic engineering and biotechnology as they enable the precise cutting and manipulation of DNA.
- The ability to cut DNA at specific locations allows for the insertion of genes or DNA fragments into other DNA molecules, such as plasmids or chromosomes.
- This technology is crucial for creating recombinant DNA molecules, which are widely used in various applications, including the production of pharmaceuticals, gene therapy, and agriculture.
- Restriction enzymes also play a role in DNA fingerprinting, a technique used for forensic analysis and paternity testing. By cutting DNA with different restriction enzymes, unique DNA fragment patterns can be produced, which can be used for identification purposes.

Conclusion:
In summary, the discovery of restriction enzymes revolutionized the field of biotechnology by enabling the precise cutting of DNA at specific locations. This breakthrough allowed scientists to manipulate DNA fragments and create recombinant DNA molecules, leading to numerous applications in medicine, agriculture, and forensic science.
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B
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Read the following text and answer the following question on the basis of the same:The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.Q. The cutting of DNA at specific locations became possible with the discovery of :a)Ligasesb)Restriction enzymesc)Probesd)Selectable markers.Correct answer is option 'B'. Can you explain this answer?
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Read the following text and answer the following question on the basis of the same:The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.Q. The cutting of DNA at specific locations became possible with the discovery of :a)Ligasesb)Restriction enzymesc)Probesd)Selectable markers.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Read the following text and answer the following question on the basis of the same:The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.Q. The cutting of DNA at specific locations became possible with the discovery of :a)Ligasesb)Restriction enzymesc)Probesd)Selectable markers.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Read the following text and answer the following question on the basis of the same:The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.Q. The cutting of DNA at specific locations became possible with the discovery of :a)Ligasesb)Restriction enzymesc)Probesd)Selectable markers.Correct answer is option 'B'. Can you explain this answer?.
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