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Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :
Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.
Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.
- a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).
- b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).
- c)Assertion (A) is true but reason (R) is false.
- d)Assertion (A) is false but reason (R) is true.
Correct answer is option 'A'. Can you explain this answer?
Verified Answer
Directions : In the following questions a statement of assertion (A) ...
PBR322 carries recognition sites for a number of commonly used restriction enzymes. Recognition site for BamHl is present in the tetr region i.e., the region responsible for tetracycline resistance. When an insert is added at the BamHl recognition site the gene for tetracycline resistance becomes non-functional and the recombinant bacteria with plasmid pBR322 that has DNA inserted at BamHl lose tetracycline resistance.
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Directions : In the following questions a statement of assertion (A) ...
Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.
Reason (R) : Recognition site for BamH I is present in TetR region of pBR322.
The correct answer is option 'A', which states that both the assertion (A) and the reason (R) are true and the reason (R) is the correct explanation of the assertion (A).
Explanation:
Understanding Assertion (A):
The assertion states that E. coli having pBR322 with DNA insert at the BamHI site cannot grow in a medium containing tetracycline.
Understanding Reason (R):
The reason states that the recognition site for BamHI is present in the TetR region of pBR322.
Explanation of Assertion (A) and Reason (R):
The pBR322 plasmid is a commonly used cloning vector in molecular biology. It contains two genes, TetR and AmpR, which confer resistance to tetracycline and ampicillin, respectively. These antibiotic resistance genes are flanked by restriction enzyme recognition sites, including the BamHI site.
When a DNA insert is introduced into the BamHI site of pBR322, it disrupts the TetR gene. This means that the E. coli cells containing pBR322 with a DNA insert at the BamHI site will not be able to produce the TetR protein, which is necessary for resistance to tetracycline.
The recognition site for BamHI is indeed present in the TetR region of pBR322. BamHI is a restriction enzyme that recognizes the DNA sequence GGATCC and cuts between the two G's. This recognition site is located within the TetR gene of pBR322.
Therefore, when a DNA insert is introduced at the BamHI site, it disrupts the TetR gene and renders the E. coli cells unable to produce the TetR protein. As a result, these cells cannot grow in a medium containing tetracycline, as they do not have the necessary resistance to the antibiotic.
Conclusion:
Both the assertion (A) and reason (R) are true, and the reason (R) correctly explains why E. coli with pBR322 and a DNA insert at the BamHI site cannot grow in a medium containing tetracycline.
Reason (R) : Recognition site for BamH I is present in TetR region of pBR322.
The correct answer is option 'A', which states that both the assertion (A) and the reason (R) are true and the reason (R) is the correct explanation of the assertion (A).
Explanation:
Understanding Assertion (A):
The assertion states that E. coli having pBR322 with DNA insert at the BamHI site cannot grow in a medium containing tetracycline.
Understanding Reason (R):
The reason states that the recognition site for BamHI is present in the TetR region of pBR322.
Explanation of Assertion (A) and Reason (R):
The pBR322 plasmid is a commonly used cloning vector in molecular biology. It contains two genes, TetR and AmpR, which confer resistance to tetracycline and ampicillin, respectively. These antibiotic resistance genes are flanked by restriction enzyme recognition sites, including the BamHI site.
When a DNA insert is introduced into the BamHI site of pBR322, it disrupts the TetR gene. This means that the E. coli cells containing pBR322 with a DNA insert at the BamHI site will not be able to produce the TetR protein, which is necessary for resistance to tetracycline.
The recognition site for BamHI is indeed present in the TetR region of pBR322. BamHI is a restriction enzyme that recognizes the DNA sequence GGATCC and cuts between the two G's. This recognition site is located within the TetR gene of pBR322.
Therefore, when a DNA insert is introduced at the BamHI site, it disrupts the TetR gene and renders the E. coli cells unable to produce the TetR protein. As a result, these cells cannot grow in a medium containing tetracycline, as they do not have the necessary resistance to the antibiotic.
Conclusion:
Both the assertion (A) and reason (R) are true, and the reason (R) correctly explains why E. coli with pBR322 and a DNA insert at the BamHI site cannot grow in a medium containing tetracycline.
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Directions : In the following questions a statement of assertion (A) ...
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Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer?.
Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer?.
Solutions for Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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Here you can find the meaning of Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of
Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer?, a detailed solution for Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? has been provided alongside types of Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'A'. Can you explain this answer? tests, examples and also practice NEET tests.
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