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Read the following and answer the question given below:
Rohan, a 13 year old school student has been diagnosed with AIDS. He often complains of fever, nausea, headache and lethargy. Doctor advised some drugs to prolong his life.
AIDS cannot be diagnosed by
  • a)
    ELISA
  • b)
    PCR
  • c)
    Western blotting
  • d)
    VDRL
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
Read the following and answer the question given below:Rohan, a 13 ye...
AIDS can be diagnosed by ELISA test and western blotting test. Western blotting test is used for confirmation of ELISA positive cases. PCR is also used to diagnose AIDS. VDRL (Venereal Disease Research Laboratory) is not used to detect AIDS.
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Most Upvoted Answer
Read the following and answer the question given below:Rohan, a 13 ye...
Answer:

Introduction

In the given scenario, Rohan, a 13-year-old school student, has been diagnosed with AIDS. He experiences symptoms such as fever, nausea, headache, and lethargy. The doctor has prescribed certain drugs to prolong his life. The question asks for the diagnostic test that cannot be used to diagnose AIDS.

Explanation

ELISA:
ELISA stands for Enzyme-Linked Immunosorbent Assay. It is a commonly used diagnostic test to detect the presence of antibodies or antigens in a person's blood. In the case of AIDS, ELISA is used to detect the presence of HIV antibodies. Therefore, ELISA can be used to diagnose AIDS.

PCR:
PCR stands for Polymerase Chain Reaction. It is a molecular technique used to amplify a specific segment of DNA. In the case of AIDS, PCR can be used to detect the presence of the HIV virus in a person's blood. Therefore, PCR can be used to diagnose AIDS.

Western Blotting:
Western blotting is a technique used to detect specific proteins in a sample. In the case of AIDS, Western blotting is used to confirm the presence of HIV antibodies after a positive ELISA test. It helps to differentiate between false-positive and true-positive results. Therefore, Western blotting can be used to diagnose AIDS.

VDRL:
VDRL stands for Venereal Disease Research Laboratory. It is a blood test used to detect the presence of antibodies against the bacteria that cause syphilis. Syphilis is a sexually transmitted infection and is not related to AIDS. Therefore, VDRL cannot be used to diagnose AIDS.

Conclusion

In conclusion, among the given options, the diagnostic test that cannot be used to diagnose AIDS is VDRL. ELISA, PCR, and Western blotting are all valid diagnostic tests used in the detection and confirmation of AIDS.
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Read the following and answer the question given below:Rohan, a 13 year old school student has been diagnosed with AIDS. He often complains of fever, nausea, headache and lethargy. Doctor advised some drugs to prolong his life.AIDS cannot be diagnosed bya)ELISAb)PCRc)Western blottingd)VDRLCorrect answer is option 'D'. Can you explain this answer?
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