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Eukaryotic genes do not function properly when cloned into a bacterial cell because
- a)of the inability to excise introns and destruction by bacterial restriction enzymes
- b)of high pH present in bacterial cells
- c)of inappropriate insertion of genes
- d)both (a) and (b)
Correct answer is option 'A'. Can you explain this answer?
Most Upvoted Answer
Eukaryotic genes do not function properly when cloned into a bacterial...
Eukaryotic genes do not function properly When transferred into bacterial cell because introns are present eukaryotic cells but are absent in prokaryotic cells. Hence, when bacterial cell is transformed with recombinant DNA is genrated human gene, it could not process it. As a result no desired protien will be produced.
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Eukaryotic genes do not function properly when cloned into a bacterial...
Inability to excise introns:
Eukaryotic genes contain introns, which are non-coding regions of DNA that interrupt the coding regions called exons. Before the gene is transcribed into mRNA, these introns must be removed through a process called splicing. Bacterial cells lack the machinery required for splicing and therefore cannot remove introns from eukaryotic genes. This means that if a eukaryotic gene is cloned into a bacterial cell, the introns will remain in the mRNA, leading to the production of non-functional proteins.
Destruction by bacterial restriction enzymes:
Bacterial cells possess a defense mechanism against foreign DNA called restriction enzymes. These enzymes recognize specific DNA sequences and cleave the DNA at those sites. Eukaryotic genes cloned into bacterial cells may contain DNA sequences that are recognized by bacterial restriction enzymes, resulting in the destruction of the cloned gene.
Both (a) and (b):
Therefore, the correct answer is option (d), both (a) and (b), as both the inability to excise introns and destruction by bacterial restriction enzymes contribute to the improper functioning of eukaryotic genes when cloned into bacterial cells.
In summary, eukaryotic genes do not function properly when cloned into bacterial cells because bacterial cells lack the ability to excise introns and may destroy the cloned gene through the action of restriction enzymes. This highlights the fundamental differences between eukaryotic and bacterial gene expression mechanisms, making it necessary to use alternative methods, such as using eukaryotic expression systems, to successfully express and study eukaryotic genes.
Eukaryotic genes contain introns, which are non-coding regions of DNA that interrupt the coding regions called exons. Before the gene is transcribed into mRNA, these introns must be removed through a process called splicing. Bacterial cells lack the machinery required for splicing and therefore cannot remove introns from eukaryotic genes. This means that if a eukaryotic gene is cloned into a bacterial cell, the introns will remain in the mRNA, leading to the production of non-functional proteins.
Destruction by bacterial restriction enzymes:
Bacterial cells possess a defense mechanism against foreign DNA called restriction enzymes. These enzymes recognize specific DNA sequences and cleave the DNA at those sites. Eukaryotic genes cloned into bacterial cells may contain DNA sequences that are recognized by bacterial restriction enzymes, resulting in the destruction of the cloned gene.
Both (a) and (b):
Therefore, the correct answer is option (d), both (a) and (b), as both the inability to excise introns and destruction by bacterial restriction enzymes contribute to the improper functioning of eukaryotic genes when cloned into bacterial cells.
In summary, eukaryotic genes do not function properly when cloned into bacterial cells because bacterial cells lack the ability to excise introns and may destroy the cloned gene through the action of restriction enzymes. This highlights the fundamental differences between eukaryotic and bacterial gene expression mechanisms, making it necessary to use alternative methods, such as using eukaryotic expression systems, to successfully express and study eukaryotic genes.
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Eukaryotic genes do not function properly when cloned into a bacterial cell becausea)of the inability to excise introns and destruction by bacterial restriction enzymesb)of high pH present in bacterial cellsc)of inappropriate insertion of genesd)both (a) and (b)Correct answer is option 'A'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Eukaryotic genes do not function properly when cloned into a bacterial cell becausea)of the inability to excise introns and destruction by bacterial restriction enzymesb)of high pH present in bacterial cellsc)of inappropriate insertion of genesd)both (a) and (b)Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Eukaryotic genes do not function properly when cloned into a bacterial cell becausea)of the inability to excise introns and destruction by bacterial restriction enzymesb)of high pH present in bacterial cellsc)of inappropriate insertion of genesd)both (a) and (b)Correct answer is option 'A'. Can you explain this answer?.
Eukaryotic genes do not function properly when cloned into a bacterial cell becausea)of the inability to excise introns and destruction by bacterial restriction enzymesb)of high pH present in bacterial cellsc)of inappropriate insertion of genesd)both (a) and (b)Correct answer is option 'A'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Eukaryotic genes do not function properly when cloned into a bacterial cell becausea)of the inability to excise introns and destruction by bacterial restriction enzymesb)of high pH present in bacterial cellsc)of inappropriate insertion of genesd)both (a) and (b)Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Eukaryotic genes do not function properly when cloned into a bacterial cell becausea)of the inability to excise introns and destruction by bacterial restriction enzymesb)of high pH present in bacterial cellsc)of inappropriate insertion of genesd)both (a) and (b)Correct answer is option 'A'. Can you explain this answer?.
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