Read statements (i)-(iv). Which of the following statements are incorr...
In 1997, Rosie, the tirst transgenic cow was engineered to produce milk enriched with a human protein called alpha-lactalbumin, making it nutritionally more balanced. Restriction enzymes are used to cut DNA at specific sites.
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Read statements (i)-(iv). Which of the following statements are incorr...
Explanation:
The incorrect statements among (i)-(iv) are:
(i) First transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.
(ii) Restriction enzymes are used in isolation of DNA from other macromolecules.
Explanation of Incorrect Statement (i):
The first transgenic buffalo Rosie was not produced to produce milk which was human alpha-lactalbumin enriched. It was actually the first transgenic cow named Rosie that was created to produce milk enriched in human alpha-lactalbumin. This milk had potential therapeutic applications for infants who were unable to digest regular cow's milk.
Explanation of Incorrect Statement (ii):
Restriction enzymes are not used in the isolation of DNA from other macromolecules. Instead, they are used in the process of cutting DNA at specific recognition sites. These enzymes recognize specific DNA sequences and cleave the DNA at or near these sites. This property of restriction enzymes is utilized in various applications of recombinant DNA technology, such as cloning and gene expression.
Explanation of Correct Statements (iii) and (iv):
(iii) Downstream processing is one of the steps of rDNA technology.
Downstream processing refers to the purification and separation of the desired product (e.g., recombinant protein) from the mixture of various cellular components. It is an essential step in rDNA technology to obtain a pure and concentrated product.
(iv) Disarmed pathogen vectors are also used in the transfer of rDNA into the host.
Disarmed pathogen vectors, which are modified versions of pathogenic organisms, are commonly used in the transfer of recombinant DNA into the host organism. These vectors have their ability to cause disease removed or minimized, making them safer for use in research and biotechnology applications.
Therefore, the incorrect statements are (i) and (ii), and the correct answer is option (d) - (i) and (ii).
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