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The restriction enzyme responsible for the cleavage of following sequence  is 

5' - G - T - C - G - A - c - 3'

3' - C - A - G - C - T - G - 5'

  • a)
    Eco RI

  • b)
    Hind II

  • c)
    Bam HI

  • d)
    Alu I

Correct answer is option 'B'. Can you explain this answer?
Most Upvoted Answer
The restriction enzyme responsible for the cleavage of following seque...
HindII was the first discovered restriction endonuclease. It was isolated from Haemophilus influenzae. It produces blunt ends.
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The restriction enzyme responsible for the cleavage of following seque...
Explanation:


The sequence given is:


5'-G-T-C-G-A-c-3'


3'-C-A-G-C-T-G-5'


The restriction enzyme responsible for the cleavage of the given sequence is Hind II.


Reason:


Hind II cleaves DNA at the following sequence:


5'-A-N-GCT-3'


3'-T-N-CGA-5'


Here, N represents any nucleotide.


On comparing the given sequence and the recognition sequence of Hind II, we can see that the given sequence has the recognition sequence of Hind II in its complementary strand.


Therefore, Hind II can cleave the given sequence at the following site:


5'- G-T-C-G-A- C -3'


3'- C-A-G-C-T-G- G -5'


Hence, the correct answer is option B, Hind II.
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