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If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.
- a)Selectable marker
- b)Recombinant protein
- c)Cloning site
- d)Chemical scalpels
Correct answer is option 'A'. Can you explain this answer?
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If a recombinant DNA bearing gene for resistance to antibiotic ampicil...
The cloning vector requires the presence of a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc. are considered useful selectable markers for E. coli. The normal E.coli cells do not carry resistance against any of these antibiotics.
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If a recombinant DNA bearing gene for resistance to antibiotic ampicil...
Selectable Marker as a Recombinant DNA
Selectable markers are genes that confer resistance to specific antibiotics or herbicides, which allow the selection and identification of cells that have been transformed with recombinant DNA. In the case of transferring a gene for resistance to the antibiotic ampicillin into E. coli cells, the host cells become transformed into ampicillin-resistant cells. The ampicillin-resistant gene in this case is a selectable marker.
Explanation:
Recombinant DNA technology involves the manipulation of DNA molecules, and the introduction of foreign DNA sequences into host cells. The foreign DNA sequence can be a gene from another organism, a synthetic DNA fragment, or a modified version of a native gene. The goal of recombinant DNA technology is to express and study the function of specific genes, or to produce proteins of interest.
Selectable markers are essential tools for recombinant DNA technology, as they allow the identification and selection of cells that have taken up the recombinant DNA. Selectable markers can be antibiotic resistance genes, herbicide resistance genes, or fluorescent proteins. In the case of ampicillin resistance, the recombinant DNA can be introduced into E. coli cells, which will become transformed into ampicillin-resistant cells. When these cells are grown on agar plates containing ampicillin, only the transformants will grow, and the non-transformed recipient cells will die. This allows the identification of cells that have taken up the recombinant DNA.
Selectable markers are also important for the production of recombinant proteins. Many expression systems use selectable markers to select cells that have stably integrated the recombinant DNA, and to ensure the expression of the recombinant protein. For example, a selectable marker such as neomycin resistance can be used to select cells that have taken up the recombinant DNA, and a promoter sequence can be used to drive the expression of the recombinant protein.
Conclusion:
Therefore, in the context of recombinant DNA technology, a selectable marker is a gene that confers resistance to specific antibiotics or herbicides, which allows the selection and identification of cells that have been transformed with recombinant DNA. In the case of transferring a gene for resistance to the antibiotic ampicillin into E. coli cells, the ampicillin-resistant gene is a selectable marker.
Selectable markers are genes that confer resistance to specific antibiotics or herbicides, which allow the selection and identification of cells that have been transformed with recombinant DNA. In the case of transferring a gene for resistance to the antibiotic ampicillin into E. coli cells, the host cells become transformed into ampicillin-resistant cells. The ampicillin-resistant gene in this case is a selectable marker.
Explanation:
Recombinant DNA technology involves the manipulation of DNA molecules, and the introduction of foreign DNA sequences into host cells. The foreign DNA sequence can be a gene from another organism, a synthetic DNA fragment, or a modified version of a native gene. The goal of recombinant DNA technology is to express and study the function of specific genes, or to produce proteins of interest.
Selectable markers are essential tools for recombinant DNA technology, as they allow the identification and selection of cells that have taken up the recombinant DNA. Selectable markers can be antibiotic resistance genes, herbicide resistance genes, or fluorescent proteins. In the case of ampicillin resistance, the recombinant DNA can be introduced into E. coli cells, which will become transformed into ampicillin-resistant cells. When these cells are grown on agar plates containing ampicillin, only the transformants will grow, and the non-transformed recipient cells will die. This allows the identification of cells that have taken up the recombinant DNA.
Selectable markers are also important for the production of recombinant proteins. Many expression systems use selectable markers to select cells that have stably integrated the recombinant DNA, and to ensure the expression of the recombinant protein. For example, a selectable marker such as neomycin resistance can be used to select cells that have taken up the recombinant DNA, and a promoter sequence can be used to drive the expression of the recombinant protein.
Conclusion:
Therefore, in the context of recombinant DNA technology, a selectable marker is a gene that confers resistance to specific antibiotics or herbicides, which allows the selection and identification of cells that have been transformed with recombinant DNA. In the case of transferring a gene for resistance to the antibiotic ampicillin into E. coli cells, the ampicillin-resistant gene is a selectable marker.
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If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred toE.colicells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.a)Selectable markerb)Recombinant proteinc)Cloning sited)Chemical scalpelsCorrect answer is option 'A'. Can you explain this answer?
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If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred toE.colicells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.a)Selectable markerb)Recombinant proteinc)Cloning sited)Chemical scalpelsCorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred toE.colicells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.a)Selectable markerb)Recombinant proteinc)Cloning sited)Chemical scalpelsCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred toE.colicells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.a)Selectable markerb)Recombinant proteinc)Cloning sited)Chemical scalpelsCorrect answer is option 'A'. Can you explain this answer?.
If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred toE.colicells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.a)Selectable markerb)Recombinant proteinc)Cloning sited)Chemical scalpelsCorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred toE.colicells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.a)Selectable markerb)Recombinant proteinc)Cloning sited)Chemical scalpelsCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred toE.colicells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.a)Selectable markerb)Recombinant proteinc)Cloning sited)Chemical scalpelsCorrect answer is option 'A'. Can you explain this answer?.
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