Microinjection Method of Gene Transfer

The microinjection method is a widely used technique for introducing foreign DNA into cells. It involves the direct injection of genetic material into the nucleus of a target cell using a micro-pipette. This method allows precise control over the delivery of DNA and can be used for both single-cell and multicellular organisms.

Required Components for Microinjection Method

The following components are required for the microinjection method of gene transfer:

1. Micro-pipettes: Micro-pipettes are thin glass needles with a fine tip that is used for injecting DNA into the cell. These micro-pipettes are carefully fabricated to have a diameter small enough to penetrate the cell membrane without causing significant damage. The tip of the micro-pipette is filled with the DNA solution, and a small amount of pressure is applied to inject the DNA into the cell.

2. Micro-particles: Micro-particles are not required for the microinjection method of gene transfer. Therefore, option A is incorrect.

3. Divalent Cations: Divalent cations, such as calcium ions, are often used to enhance the efficiency of the microinjection process. These cations help to destabilize the cell membrane, making it more permeable to the injected DNA. However, they are not essential for the microinjection method itself. Therefore, option C is incorrect.

4. UV Radiations: UV radiations are not required for the microinjection method of gene transfer. Therefore, option D is incorrect.

Conclusion

In conclusion, the only component required for the microinjection method of gene transfer is micro-pipettes. Micro-pipettes are used to inject DNA into the nucleus of the target cell. Micro-particles, divalent cations, and UV radiations are not necessary for this method.