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In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of
- a)Silver or Tungsten
- b)Arsenic or Silver
- c)Gold or Tungsten
- d)None of these
Correct answer is option 'C'. Can you explain this answer?
Verified Answer
In biolistic method of gene transfer, the microparticles coated with f...
Biolistic method or gene gun method is a direct or vectorless method of introducing DNA into cells that involves bombardment of cells with high-velocity microprojectiles coated with DNA. In biolistic method, tungsten or gold particles, coated with foreign DNA are bombarded into target at a very high velocity.
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In biolistic method of gene transfer, the microparticles coated with f...
Biolistic Method of Gene Transfer
The biolistic method, also known as the "gene gun" method, is a technique used to introduce foreign DNA into target cells. This process is particularly useful in plant biotechnology and genetic engineering.
Microparticles Used
- Microparticles in this method are typically made of Gold or Tungsten.
- These metals are chosen due to their high density and ability to penetrate cell walls effectively.
Process of Gene Transfer
- Coating with DNA: Foreign DNA is coated onto the surface of these microparticles.
- High Velocity Bombardment: The microparticles are then propelled at a very high velocity towards the target cells using a burst of helium gas or a similar mechanism.
- Cell Penetration: The high kinetic energy allows the microparticles to penetrate the cell membrane, delivering the DNA directly into the cell's cytoplasm.
Advantages of Using Gold or Tungsten
- Biocompatibility: Both gold and tungsten are biocompatible, reducing the chances of eliciting an immune response.
- Efficient Delivery: Their density allows for efficient delivery of the genetic material, increasing the likelihood of successful transformation.
Conclusion
In summary, the correct answer to the question regarding the materials used for microparticles in the biolistic method of gene transfer is option C: Gold or Tungsten. These materials are specifically chosen for their properties that facilitate effective gene delivery into target cells.
The biolistic method, also known as the "gene gun" method, is a technique used to introduce foreign DNA into target cells. This process is particularly useful in plant biotechnology and genetic engineering.
Microparticles Used
- Microparticles in this method are typically made of Gold or Tungsten.
- These metals are chosen due to their high density and ability to penetrate cell walls effectively.
Process of Gene Transfer
- Coating with DNA: Foreign DNA is coated onto the surface of these microparticles.
- High Velocity Bombardment: The microparticles are then propelled at a very high velocity towards the target cells using a burst of helium gas or a similar mechanism.
- Cell Penetration: The high kinetic energy allows the microparticles to penetrate the cell membrane, delivering the DNA directly into the cell's cytoplasm.
Advantages of Using Gold or Tungsten
- Biocompatibility: Both gold and tungsten are biocompatible, reducing the chances of eliciting an immune response.
- Efficient Delivery: Their density allows for efficient delivery of the genetic material, increasing the likelihood of successful transformation.
Conclusion
In summary, the correct answer to the question regarding the materials used for microparticles in the biolistic method of gene transfer is option C: Gold or Tungsten. These materials are specifically chosen for their properties that facilitate effective gene delivery into target cells.
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In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up ofa)Silver or Tungstenb)Arsenic or Silverc)Gold or Tungstend)None of theseCorrect answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up ofa)Silver or Tungstenb)Arsenic or Silverc)Gold or Tungstend)None of theseCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up ofa)Silver or Tungstenb)Arsenic or Silverc)Gold or Tungstend)None of theseCorrect answer is option 'C'. Can you explain this answer?.
In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up ofa)Silver or Tungstenb)Arsenic or Silverc)Gold or Tungstend)None of theseCorrect answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up ofa)Silver or Tungstenb)Arsenic or Silverc)Gold or Tungstend)None of theseCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up ofa)Silver or Tungstenb)Arsenic or Silverc)Gold or Tungstend)None of theseCorrect answer is option 'C'. Can you explain this answer?.
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