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Read the given statements and select the correct option.
Statement 1:
The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.
Statement 2: Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.
  • a)
    Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.
  • b)
    Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.
  • c)
    Statement 1 is correct and statement 2 is incorrect.
  • d)
    Both statements 1 and 2 are incorrect. 
Correct answer is option 'A'. Can you explain this answer?
Verified Answer
Read the given statements and select the correct option.Statement 1: T...
When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' produced, which can be joined together (end-to-end) using DNA ligase. Restriction enzymes are of two kinds - exonucleases and endonucleases. Exonucleases remove nucleotides from the j ends of the DNA whereas endonucleases make cuts at specific positions within the DNA. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. Therefore, in order to link the alien DNA (or foreign DNA), the vector needs to have very few, preferably single, recognition/cloning sites for the commonly used restriction enzymes.
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Most Upvoted Answer
Read the given statements and select the correct option.Statement 1: T...
Understanding Cloning Vectors
Cloning vectors play a crucial role in genetic engineering, allowing scientists to insert and replicate DNA fragments. Let's break down the provided statements to understand their correctness.
Statement 1: Cloning Vector Characteristics
- Cloning vectors are designed to have minimal recognition sites for restriction enzymes.
- The ideal scenario is to have preferably a single recognition site to simplify the cloning process.
Statement 2: Impact of Multiple Recognition Sites
- If a cloning vector has more than one recognition site for restriction enzymes, it will result in the generation of multiple DNA fragments upon restriction digestion.
- This complexity can hinder the gene cloning process, making it more challenging to isolate and manipulate the desired fragment.
Conclusion: Explanation of the Correct Option
- Both statements are correct. Statement 1 accurately describes the ideal characteristics of a cloning vector.
- Statement 2 provides a valid explanation for why having multiple recognition sites complicates gene cloning, reinforcing the importance of having few recognition sites.
Thus, the correct answer is option 'A': Both statements 1 and 2 are correct, and statement 2 is the correct explanation of statement 1.
This comprehension of cloning vectors is essential for students and professionals in the field of biotechnology and genetics.
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Read the given statements and select the correct option.Statement 1: The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.Statement 2: Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.a)Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.b)Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.c)Statement 1 is correct and statement 2 is incorrect.d)Both statements 1 and 2 are incorrect.Correct answer is option 'A'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Read the given statements and select the correct option.Statement 1: The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.Statement 2: Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.a)Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.b)Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.c)Statement 1 is correct and statement 2 is incorrect.d)Both statements 1 and 2 are incorrect.Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Read the given statements and select the correct option.Statement 1: The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.Statement 2: Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.a)Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.b)Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.c)Statement 1 is correct and statement 2 is incorrect.d)Both statements 1 and 2 are incorrect.Correct answer is option 'A'. Can you explain this answer?.
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