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To prove that DNA is the genetic material, which radioactive isotopes were used by Hershey and Chase(1952) in their experiments?
  • a)
    35S and 15N
  • b)
    32P and 35S
  • c)
    32P and 15N
  • d)
    14N and 5N
Correct answer is option 'B'. Can you explain this answer?
Verified Answer
To prove that DNA is the genetic material, which radioactive isotopes ...
Hershey and Chase experiment is based on the fact that DNA contains phosphorus and similarly, sulphur is present in proteins but not in DNA. They incorporated radioactive isotope of phosphorus (32P) into phage DNA and that of sulphur (35S) into proteins of a separate phage culture. Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but proteins do not, Similarly, viruses grown on radioactive sulphur contain radioactive- protein but not-radioactive DNA because DNA does not contain sulphur.
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Most Upvoted Answer
To prove that DNA is the genetic material, which radioactive isotopes ...
Hershey and Chase used radioactive isotopes to prove that DNA is the genetic material. They used bacteriophages (viruses that infect bacteria) to carry out their experiments.

The two radioactive isotopes used by Hershey and Chase were:

- 32P (radioactive phosphorus): This is a radioactive isotope of phosphorus that can be incorporated into the DNA molecule.
- 35S (radioactive sulfur): This is a radioactive isotope of sulfur that can be incorporated into the protein coat of the bacteriophage.

Explanation:

Hershey and Chase conducted an experiment where they labeled the DNA of the bacteriophage with 32P and the protein coat with 35S. They allowed the bacteriophage to infect E. coli bacteria and then separated the bacteriophage from the bacteria using a blender and a centrifuge.

They found that almost all of the 32P (radioactive DNA) was found inside the bacteria, while most of the 35S (radioactive protein) remained outside. This suggested that the DNA of the bacteriophage was responsible for carrying the genetic information that was passed on to the bacteria.

Therefore, the use of 32P and 35S in the Hershey and Chase experiment helped to prove that DNA is the genetic material.
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To prove that DNA is the genetic material, which radioactive isotopes were used by Hershey and Chase(1952) in their experiments?a)35S and 15Nb)32P and 35Sc)32P and 15Nd)14N and 5NCorrect answer is option 'B'. Can you explain this answer?
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