In an experiment to prepare the temporary mount of a leaf peel to show stomata ,why glycerine and safranin are used?

Question

Answer

Leaf peel is transparent,so the cells will also appear transparent under the microscope. to see the cells clearly, Safranin is used in the preparation of slide.

Answer

Glycerine is used to avoid the dryness of leaf peel. glycerine is also called Vaseline.saffarin solution is used to see the leaf peel distinctly under microscope because leaf peel structures are transparent

Answer

Introduction:
In an experiment to prepare a temporary mount of a leaf peel to show stomata, glycerine and safranin are commonly used. These substances serve specific purposes and help in the visualization and preservation of stomata on the leaf surface.

Reasons for using glycerine:
1. Clearing agent: Glycerine acts as a clearing agent, meaning it helps in making the leaf transparent by removing chlorophyll and other pigments. This transparency allows for better observation of the stomata under a microscope.
2. Refractive index matching: Glycerine has a refractive index close to that of glass, which helps in reducing the refraction of light as it passes through the leaf peel. This reduces distortion and improves the clarity of the stomata image.

Reasons for using safranin:
1. Staining agent: Safranin is a red dye that is used to stain the cells of the leaf peel. It selectively stains certain structures, including the nuclei and cell walls, making them more visible under a microscope. This staining allows for easier identification and counting of the stomata.
2. Contrast enhancement: Safranin enhances the contrast between different cellular components, making it easier to distinguish the stomata from the surrounding cells. This aids in accurate observation and measurement of stomatal characteristics such as size, shape, and density.

The procedure:
1. Obtaining a leaf peel: A small piece of the leaf is carefully detached using forceps or a scalpel. The leaf is preferably from the lower epidermis, as it typically has a higher density of stomata.
2. Clearing the leaf peel: The leaf peel is immersed in a solution of glycerine for a few minutes. Glycerine penetrates the cells, removing chlorophyll and other pigments, and making the peel transparent.
3. Staining with safranin: The leaf peel is then transferred to a safranin solution for a brief period, usually a few seconds to a minute. The safranin stains the cells, including the nuclei and cell walls, giving them a reddish color.
4. Mounting the peel: The stained leaf peel is carefully placed on a glass slide, preferably with a drop of glycerine. A cover slip is gently placed on top, ensuring there are no air bubbles.
5. Observation: The mounted leaf peel is observed under a microscope, using appropriate magnification and lighting. The stomata should now be clearly visible, appearing as small openings surrounded by stained cells.

Conclusion:
Glycerine and safranin play crucial roles in preparing a temporary mount of a leaf peel to show stomata. Glycerine acts as a clearing agent, making the peel transparent and reducing refraction, while safranin acts as a staining agent, enhancing contrast and making the stomata and surrounding cells more visible. These steps allow for accurate observation and analysis of stomatal characteristics, contributing to a deeper understanding of plant physiology and adaptation.

Similar Class 10 Doubts

In the experiment of preparing a temporary mount of a leaf peel to observe stomata, we use two liquids other than water. Name these two liquids and state when and why these liquids are used.?

In an experiment to prepare a temporary mount of leaf peel staining is done before putting a drop of glycerin?why?

List the three steps of preparation of temporary mount of leaf peel to observe stomata?

What are stomata ? Write two function of stomata ?

Top Courses for Class 10View all

Science Class 10

Social Studies (SST) Class 10

Mathematics (Maths) Class 10

English Grammar Advanced

English Grammar Basic

Top Courses for Class 10

Top Courses for Class 10

Suggested Free Tests

Related Content

About Class 10

Class 10 Study Material

Class 10 All Subjects

NCERT Textbook Solutions

Other Classes

Important Links

Company