Meristem Culture:
Meristem culture is the best method to obtain virus-free plants through tissue culture. Meristems are the actively dividing regions of plant tissue where the growth and development of the plant occur. These regions are free from viral infection, making them ideal for obtaining virus-free plants.

Process:
The process of meristem culture involves the following steps:

1. Selection of Healthy Plant: A healthy plant showing symptoms of viral infection is selected as the source plant.

2. Sterilization: The plant material (usually shoot tips or buds) is sterilized to remove any surface contaminants. This is done by using a combination of disinfectants such as bleach or ethanol.

3. Isolation of Meristem: After sterilization, the shoot tip or bud is dissected to isolate the meristem region. The meristem is the tiny, actively dividing region at the tip of the shoot that contains undifferentiated cells.

4. Culture in Nutrient Medium: The isolated meristem is then placed in a nutrient medium containing growth regulators such as cytokinins and auxins. The medium provides essential nutrients and hormones for the growth of the meristem.

5. Shoot Multiplication: The meristem starts to multiply and produce multiple shoots. These shoots are later transferred to a fresh medium to promote further growth and multiplication.

6. Rooting: Once an adequate number of shoots have been obtained, they are transferred to a rooting medium to induce root formation.

7. Acclimatization: Finally, the rooted plantlets are transferred to soil or a greenhouse environment where they are acclimatized to natural conditions.

Advantages of Meristem Culture:

- Virus-Free Plants: Meristem culture ensures the production of virus-free plants as the meristem tissue is free from viral infection.

- Rapid Multiplication: The meristem tissue has a high multiplication rate, allowing for rapid production of numerous plantlets.

- Genetic Stability: The regenerated plants through meristem culture are genetically stable and identical to the parent plant.

- Conservation of Rare and Endangered Plants: Meristem culture is also used for the conservation and propagation of rare and endangered plant species.

- Disease Eradication: Meristem culture can be used not only to eliminate viruses but also other diseases such as bacteria and fungi.

In conclusion, meristem culture is the best method to obtain virus-free plants through tissue culture due to its ability to produce genetically stable and disease-free plants.

In order to obtain virus-free plants through tissue culture, the best method is meristem culture. Meristem tip culture is used successfully to remove viruses, bacteria, in order to produce the greatest number of plants. Meristem culture is used to produce healthy propagation stock for crops and ornamentals.